| Objective:Effect of bone marrow mesenchymal stem cells(BM-MSCs)injection on wound healing of whole skin resection in mice with breast cancer was studied,which provided theoretical basis for clinical treatment of breast cancer wound.Methods:The whole bone marrow was extracted for BM-MSCs isolation and culture,and the BM-MSCs was identified from functional and morphological aspects.a mouse breast cancer model was established by 4 T1 cell line transplantation.The normal skin wound model and breast cancer skin wound model were established in the shoulder of BALB/c mice.They were randomly divided into three groups.Group A that breast cancer wound was affected by BM-MSCs,group B that breast cancer wound was affected by saline and group C that normal skin wound was affected by saline.The wound healing in each group was observed at the postoperative 7 d、14 d、21 d,photographing skin wound healing in each group,recording the wound healing rate in each group.Pathological examination the pathological changes of skin wound in each group were detected by HE staining,and the VEGF、TGF-β1 and Smad7 expression of skin wound in each group were detected by immunohistochemistry.Results:1.A large number of biologically active BM-MSCs,can be quickly separated by the whole bone marrow method Cell morphology observation and induction of lipogenesis and osteogenesis suggest that the cultured cells are BM-MSCs.The mouse inoculated with 4T1 cells had tumorigenesis on the back of the shoulder.Pathological suggested invasive breast cancer.The cell of breast cancer infiltration on the surface of wound in mice indicates that breast cancer wound modeling is successful.2.7 d after surgery,the skin wound healing in group C compared with group B,which healing was faster(P<0.05),in group A and group B the healing rate of skin wound was similar(P>0.05);At 14 dafter surgery,The healing rate of skin wound in group B was significantly slower than that in group A and group C(P<0.05),in group A and group C the healing rate of skin wound was similar(P>0.05);At 21 d after surgery,in group B compare with group A and group C the healing rate of skin wound was similar(P>0.05),The wound healing rate of group C obviously did not prevail compared with group A(P<0.05).、3.Immunohistochemistry showed that the expression of wound of VEGF、TGF-β1 in group A、group B and group C at 7 d and 14 d after surgery were increased gradually;At 21 d after surgery gradually decreasing;It is the group A that the VEGF、TGF-β1 expression was the highest,in the group B was the second,and the group C was the lowest;At 7 d、14d dafter surgery,the expression of Smad7 was decreased gradually in group A,group B and group C;At 21 d after surgery was increasing expression,And each time point,the expression of Smad7 in the group A was lowest;the group B was the second,The group C was the highest.At 7 d and 14d after surgery,The expression of VEGF in group A was higher than that in group B(p<0.05);In group C,The expression of VEGF was the same as that in group A and group B(p>0.05);At 21 d after surgery,The expression of VEGF in group A was higher than that in group B and group C(0.05);The expression of VEGF in group B was the same as that in group C(p>0.05).At 7 d after surgery,The expression of TGF-β1 and Smad7 in group A,group B and group C were equal(0.05);At 14 d after surgery,the expression of TGF-β1 in group A was the highest,followed by group B and the lowest in group C(p<0.05);The expression of Smad7 in group A was higher than that in group C(p<0.05),in group B the expression of Smad7 compare with the group A and group B were equal(P>0.05);At 21 d after surgery,The content of TGF-β1 in group A was higher than that in group C(p<0.05),The expression content of TGF-β1 in group B was the same as that in group A and group C(p>0.05);The expression of Smad7 in group A,group B and group C was equivalent.Conclusion:1.A large number of biologically active BM-MSCs,can be quickly separated by the whole bone marrow method,and can be purified by passage,can be identified by induction of adipogenesis and osteogenesis.2.The inoculated 4T1 cells could survive in mice and form tumors in the back and shoulder of the mice.The skin pathology of the mouse breast cancer wound detected breast cancer cell infiltration,indicating that the mouse breast cancer wound modeling was successful.3.Bone marrow mesenchymal stem cells can promote the wound healing of breast cancer in mice.The effect of BM-MSCs on wound healing may depend on enhancing the role of VEGF in skin wound and regulating TGF-β1/Smad7 Signaling pathway to promote angiogenesis and epithelial cell proliferation in breast cancer wound,so as to achieve skin wound repair and healing. |
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