The Detection Of Microecology And Lactobacillus Vagina In Patients With Cervical Cancer And Precancerous Lesions

[Objective]Cervical cancer is the second most common malignancy in women worldwide,after breast cancer.The vaginal microecology of normal healthy women is mainly kept stable by the physiological and anatomical structure of the Vagina,vaginal flora,local immunity and endocrine regulation,therefore,the balance of vaginal microecology is closely related to vaginal microflora and vaginal microenvironmental factors.Poor vaginal Microbiota is thought to be the cause of persistent HPV mediated cervical carcinogenesis and genital inflammation,the mechanism of which is unclear.Analysis of the cervical vaginal microenvironment may shed light on the role of hosts and microbes in the persistence of HPV into cancer.Based on vaginal microenvironment factors and vaginal Microflora,this paper analyzed the relationship among vaginal microenvironment factors,vaginal microenvironment factors,HPV and cervical lesions,it provides some evidence for regulating balance of nature,blocking HPV infection and interfering with the progression of cervical lesions.We hope to provide a new perspective for the prevention and treatment of cervical cancer from the perspective of reproductive tract microecology.[method]From July 2018 to December 2018,28 cases of normal cervical women and 23 cases of chronic inflammation of the cervix were diagnosed by pathology,there were 35 cases of Cervical intraepithelial neoplasia(CIN)and 31 cases of Cervical squamous-cell Carcinoma(SCC).The cervical risk factors were investigated face to face by structured questionnaire,and the vaginal discharge cells and exfoliated cells were collected for detection of vaginal microenvironment factors,HPV and vaginal microflora Using the 16S rRNA gene sequencing technique,we isolated and identified lactobacillus from 117 women’s Vaginas,and investigated the main species of Lactobacillus in reproductive tract of healthy women of childbearing age,cervical cancer patients and cervical intraepithelial neoplasia patients,to explore the differences of vaginal microflora and lactobacillus between women with vaginal microecological disorder and healthy women of childbearing age.[results]1.There were significant differences in occupation,nationality,parturition and HPV infection among SCC group,Cin Group,inflammation group and normal group(p<0.05).2.There were significant differences in Ph and sialidase among SCC group,Cin Group,inflammation group and normal group(p<0.05).3.There were significant differences in Nugent score,density and diversity among SCC group,Cin Group,inflammation group and normal group(p<0.05).4.There was significant difference in the diversity of vaginal flora between HPV negative group and HPV positive group(p<0.05).5.Sixteen strains of lactobacillus were isolated from the SCC group:4 Strains of Lactobacillus Vaginalis,3 strains of lactobacillus plantarum,3 strains of lactobacillus plantarum,3 strains of lactobacillus plantarum and 3 strains of Lactobacillus JENKII,three strains of lactobacillus were isolated,19 strains of lactobacillus were isolated from normal group,5 strains of lactobacillus were isolated,and 4 strains of lactobacillus were isolated.[Conclusion]1.Ph and sialylsidase are associated with cervical lesions.2.With the increase of cervical lesion grade,the density of vaginal microflora decreased and the proportion of abnormal microflora diversity increased.3.HPV positive women have a greater variety of vaginal microbes.4.The predominant bacteria groups in cervical cancer group were lactobacillus Vaginalis,lactobacillus plantarum,Lactobacillus jensenii,Lactobacillus gasseri,and in CIN group were lactobacillus cribrium,lactobacillus fermentum,Lactobacillus jensenii,Lactobacillus gasseri,Lactobacillus johnsonii.