LncRNA RBM5-AS1 Inhibits The Proliferation And Migration Of Breast Cancer Cells By Competitively Binding With MiRNA23 To Up-regulate The Expression Of RBM5

Objective: The expression and correlation of miRNA23,RBM5 and RBM5-AS1 in clinical samples were studied.The effects of miRNA23 and RBM5 on the proliferation,migration and invasion of breast cancer cells;RBM5-AS1 regulates miRNA23 and RBM5 ce RNA,so as to reveal the molecular mechanism of the antitumor effect of RBM5 and lncRNA RBM5-AS1 in the occurrence and development of breast cancer,and further improve the ce RNA network of breast cancer cells.To provide a new experimental basis and theoretical basis for the clinical treatment of breast cancer.Methods: 1.Clinical samples from 53 cases of breast cancer were collected,and the expression levels of miRNA23,RBM5 and RBM5-AS1 in breast cancer tissues were detected by q PCR,and the correlation analysis of the expression levels of miRNA23,RBM5 and RBM5-AS1 was conducted.2.To study the effects of miRNA23 and RBM5 on the proliferation,metastasis and invasion of breast cancer cells;(1)RBM5-silenced breast cancer cell lines were constructed,(2)Proliferation of RBM5-silenced breast cancer cells was detected by CCK-8 kit;(3)Invasion and migration of RBM5-silenced breast cancer cells were detected by Transwell assay;(4)The proliferation ability of RBM5-silenced breast cancer cells transfected with miRNA23 was detected by CCK-8 kit.(5)The changes of invasion and migration ability of RBM5-silenced breast cancer cells after transfection with miRNA23 were detected by Transwell assay.3.To analyze the ce RNA regulatory mechanism of RBM5-AS1 on miRNA23 and RBM5;(1)The target relationship between miRNA23 and RBM5 and RBM5-AS1 was verified by luciferase assay.(2)RBM5-AS1 was overexpressed in breast cancer cells with miRNA23 overexpression,and the m RNA expression level of RBM5 was detected by q PCR.(3)RBM5-AS1 was overexpressed in breast cancer cells with miRNA23 overexpression,and the protein expression level of RBM5 was detected by Western blot.(4)RBM5-AS1 was silenced in breast cancer cells with miRNA 23 overexpression,and the expression level of RBM5 m RNA was detected by q PCR.(5)RBM5-AS1 was silenced in breast cancer cells with miRNA23 overexpression,and the protein expression level of RBM5 was detected by Western blot.Results: In this study,the expression of RBM5 in breast cancer patients was lower than that in non-breast cancer patients through the analysis of TCGA database data.Correlation analysis of clinical samples showed that RBM5 was negatively correlated with RBM5-AS1 and miRNA23,and positively correlated with RBM5-AS1.Cell proliferation and other experimental results showed that the down-regulation of RBM5 or up-regulation of miRNA23 significantly promoted the proliferation,invasion and migration of MDA-MB-231 cells.Fluorescence quantitative PCR was used to detect the expression of RBM5 in breast cancer cells.The results showed that overexpression of miRNA23 could down-regulate the expression of RBM5 in breast cancer cells.The luciferase report showed that both RBM5-AS1 and RBM5 were the target genes of miRNA23.When the expression of RBM5-AS1 was upregulated in cells,the inhibition effect of miRNA23 on RBM5 was weakened and the expression ability of RBM5 was restored.This study preliminarily revealed the roles and interactions of RBM5,miRNA23 and lncRNA RBM5-AS1 in breast cancer,enriched the study on the pathogenesis of breast cancer,and provided an experimental basis for relevant molecules to be used as therapeutic targets for breast cancer.Conclusions:LncRNA RBM5-AS1 can competitively bind with miRNA23 to up-regulate the expression of RBM5 and inhibit the proliferation and migration of breast cancer cells.