| Objective:Pulmonary arterial hypertension is a kind of vascular remodeling of chronic diseases,the effect is not satisfied in the treatment of pulmonary arterial hypertension,treatment can not only aiming at the problem of vasoconstriction,also to inhibit proliferation and activation of reverse remodeling to solve the problem of vascular remodeling,studies show that the immune disorders in pulmonary arterial hypertension plays an important role in vascular remodeling,Th 17 and Treg cells play an important role in the immune response.At present,there are few studies on the abnormal expression of Th17 and Treg cells in multiple tissues.Therefore,in this study,we will study the changes of Treg/Th17 levels in multiple tissues of rats with pulmonary arterial hypertension induced by monocrotaline.Methods:20 male,200-250g,clean SD rats were randomly divided into two groups:normal control group(Ctr,n=10),and pulmonary arterial hypertension group(PAH,n=10).The experiment started by giving a one-time 60mg/kg monocrotaline intraperitoneal injection to establish an animal model of pulmonary arterial hypertension.Right ventricular systolic blood pressures were measured by right cardiac catheterization.WT%and W A%of pulmonary arterioles were evaluated by ipp6.0 Image Analysis Software.Right ventricular hypertrophy index(RVHI)were measured by weighing method.The proportion of Th17 and Treg cells in blood,lung,spleen and small intestine of rats in each group was measured by flow cytometry.Results:1.The right ventricular hypertrophy index and right ventricular systolic blood pressure in the pulmonary arterial hypertension group were significantly higher than those in the control group(57.76 ± 10.58 vs 26.10 ±3.98,P<0.0001,58.38±5.90 vs 18.67±2.58,P<0.0001).2.Compared with the control group,the WT%in the pulmonary arterial hypertension group was significantly higher(52.26±3.80 vs 21.13±2.41,P<0.0001),and the WA%was significantly higher(76.40±3.21 vs 38.03±3.94,P<0.0001).3.In the peripheral blood of rats,the expression of Treg cells in the pulmonary arterial hypertension group was significantly lower than that in the control group(2.35±0.29 vs 4.78±1.18,P<0.05),the expression of Th17 was significantly increased(2.78±0.53 vs 0.99±0.14,P<0.05),and the ratio of Treg/Th17 was significantly lower in the pulmonary arterial hypertension group than in the control group(1.11±0.29 vs 4.24±1.15,P<0.05).4.In the lung tissue of rats,the expression of Treg cells in the pulmonary arterial hypertension group was significantly lower than that in the control group(1.99±0.32 vs 3.46±0.17,P<0.05),the expression of Th17 was significantly increased(3.12±0.46 vs 1.28±0.31,P<0.05),and the ratio of Treg/Th17 was significantly lower in the pulmonary arterial hypertension group than in the control group(0.75±0.15 vs 3.71±1.20,P<0.05).5.In the spleen of rats,the expression of Treg cells in the pulmonary arterial hypertension group was significantly lower than that in the control group(2.62±0.38 vs 4.68±0.84,P<0.05),the expression of Th17 was significantly increased(3.23±0.31 vs 2.05±0.34,P<0.05),and the ratio of Treg/Th17 was significantly lower in the pulmonary arterial hypertension group than in the control group(1.00±0.22 vs 2.27±0.55,P<0.05).6.In the small intestine of rats,the expression of Treg cells in the pulmonary arterial hypertension group was significantly lower than that in the control group(3.32±0.47 vs 8.15 ± 1.29,P<0.05),the expression of Th17 was significantly increased(1.22±0.25 vs 0.29±0.11,P<0.05),and the ratio of Treg/Th17 was significantly lower in the pulmonary arterial hypertension group than in the control group(3.37 ± 1.28 vs 52.87±20.75,P<0.05).Conclusions:1.In the rat model of pulmonary arterial hypertension induced by monocrotaline,the right ventricular systolic pressure and right ventricular hypertrophy index increased significantly,as well as the pathological changes of pulmonary arterioles,indicating that the experimental animal model of pulmonary arterial hypertension was effectively induced.2.In monocrotaline-induced pulmonary arterial hypertension rats,there are abnormal expressions of Treg and Th 17 cells in peripheral blood,lung,spleen and small intestine,and the imbalance of Treg/Th17 may play an important role in the occurrence and development of pulmonary arterial hypertension. |
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