| Objective:To detect the expression of P38 mitogen-activated protein kinase(p38MAPK)and the excision repair cross-complementation group 1(ERCC1)in Gastric adenocarcinoma tissues,and analyze the relationship between their expressions and clinicopathological features of Gastric adenocarcinoma and their correlation,as well as the relationship between their expressions and the survival time of Gastric adenocarcinoma patients and the survival time of patients receiving chemotherapy,so as to explore new indicators for diagnosis,treatment and prognosis evaluation of Gastric cancer.Methods:Selection of North sichuan medical college affiliated hospital in July 2016 to June 2017 group,119 cases of gastric adenocarcinoma,gastric high-grade intraepithelial neoplasia 20 cases,gastric low-grade intraepithelial neoplasia 20 cases,randomly selected from 20 cases of adenocarcinoma tissues with tumor free margin.Immunohistochemical SP method was used to detect and analyze the expression of P38 and ERCC1 in gastric adenocarcinoma tissues,and the correlation between the expression of P38 and ERCC1 and various pathological changes and clinicopathological features of gastric adenocarcinoma was analyzed according to the experimental results.All experimental data were analyzed using SPSS21.0 software,and chi-square test was used for comparison between groups.Measurement data were expressed as Mean±SD.Spearman rank correlation coefficient test was used for correlation analysis.Kaplan-Meier survival analysis was performed using log-rank test for univariate analysis.P<0.05 indicated that the difference was statistically significant.Results:The expression of P38,ERCC1 was differentially expressed in gastric adenocarcinoma,gastric high-grade intraepithelial neoplasia,gastric low-grade intraepithelial neoplasia and tumor free margin,the positive expression rate in turn reduce,P38 lightning positive expression rate of 63.0%,45.0%,35.0%and 15.0%respectively,and the difference is statistically significant(squared=16.036,P<0.05);ERCC1 expression positive rate were 73.1%,40.0%,15.0%,5.0%,and the difference is statistically significant(squared=34.194,P<0.05).The positive expression of P38 and ERCC1 in gastric adenocarcinoma was correlated with the clinical stage and the depth of invasion(P<0.05),and was not correlated with the patient’s age,tumor size,lymph node metastasis or tumor differentiation degree(P>0.05).The expression of P38 and ERCC1 in gastric adenocarcinoma was positively correlated(P<0.05).The positive expression of ERCC1 was correlated with the survival time of chemotherapy patients(P<0.05).Conclusions:1 P38 and ERCC1 are differentially expressed from high to low in gastric adenocarcinoma,gastric high-grade intraepithelial neoplasia,gastric low-grade intraepithelial neoplasia,and tumor free margin,and are all related to the clinical stage and invasion depth of patients with gastric adenocarcinoma.It is speculated that P3 8 and ERCC1 are closely related to the occurrence,development and prognosis of gastric adenocarcinoma,and may be potential tumor molecular markers of gastric adenocarcinoma.2 There was a positive correlation between the expression of P38 and ERCC1 in gastric adenocarcinoma,suggesting that there was a certain correlation between the two in the mechanism of tumor regulation,and there was a mutually promoting relationship between the formation and progression of gastric adenocarcinoma and the occurrence of postoperative chemotherapy resistance.3 The positive expression of P38 and ERCC1 was not related to the overall survival prognosis of the patients,while the positive expression of P38 was not related to the survival prognosis of the patients receiving chemotherapy,while the positive expression of ERCC1 was related to the survival prognosis of the patients receiving chemotherapy,suggesting that the positive expression of P38 and ERCC1 may be used as indicators to evaluate the therapeutic effect and survival prognosis of the patients receiving chemotherapy for gastric adenocarcinoma. |
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