| Objective: Single agent chemotherapy has great side effects in the treatment of gastric cancer,and it can not be accurately delivered to the tumor site,resulting in low efficiency of chemotherapeutic drugs and many side effects.The characteristics of nanomaterials can make it a good drug loading platform and provide more choices for the delivery of chemotherapeutic drugs.Here,we developed a new type of nano-platform,which uses GOQD loaded with photosensitizer ICG and chemotherapy drug CS-6 as the core,and wraps the hybrid membrane(HM)to form the terminal material GIC@HM,which has the functions of photothermal therapy,photodynamic therapy,and chemotherapy at the same time.The biomimetic coating hybrid membrane can not only improve the safety of the nano-core,prolong its half-life,but also accurately deliver the drug to the tumor site,and improve the utilization efficiency and safety of the drug.GIC@HM can provide a safer,more efficient,and accurate treatment for gastric cancer.Methods: 1.Design and synthesis of GIC@HM.two.The hybrid membrane of the erythrocyte membrane and BGC-823 cell membrane was synthesized,and the successful synthesis of the hybrid membrane was detected by membrane fusion test and Coomassie brilliant blue staining.3.The morphology of GOQD,GIC,and GIC@HM was photographed by transmission electron microscope.4.The UV-vis absorption spectra of each component of the drug were measured.5.The particle size and surface Zeta potential of each component were measured.6.After laser irradiation,the temperature,photothermal characteristics,and photothermal stability of the materials were measured.7.The loading entrapment efficiency and release rate of the drug.8.Laser confocal scanning was used to study the time and mechanism of cellular uptake of nanomaterials.9.The drug release in vitro was detected by dialysis.10.ROS fluorescence probe was used to detect the release of ROS from cellular level materials,and ESR was used to detect the release of singlet oxygen from the materials.11.A homotypic targeting test was used to detect the targeting ability of GIC@HM to tumor cells.12.MTT cytotoxicity test was used to detect the killer cell efficiency of the material.13.Laser confocal detection of live and dead staining.14.Western blotting was used to detect the expression of apoptosis and cycle-related proteins.15.MTT was used to detect the biosafety of the materials at the cellular level.16.Hemolysis and coagulation test to detect the safety of materials in the blood.17.The local temperature changes of tumors in tumor-bearing mice were photographed by the thermal infrared camera.The fluorescence intensity of ICG was measured by 18.IVIS kinetic optical system.19.Anti-tumor experiment in vivo.20.Sections of important organs and tumors were stained with Hype and related immunohistochemistry.Results: 1.The biomimetic nano-platform GIC@HM has been successfully constructed.2.The hybrid membrane was successfully synthesized.3.GIC@HM has a stable and powerful photothermal function.4.GIC@HM can produce singlet oxygen after laser irradiation.5.MTT method proved that the material p G@HM had good biosafety,and hemolysis and coagulation test proved that GIC@HM had good biosafety.6.GIC@HM has a good targeting function.7.GIC@HM has photothermal and p H responses,and a laser and acidic environment can promote its drug release.8.The anti-tumor ability of GIC@HM nanomaterials was proved in vitro.9.The nanomaterials coated with HM have the effect of targeting and slowing down the half-life.10.In vivo experiments have proved that the combination of GIC@ HM photochemotherapy also has a strong antitumor function in vivo.11.GIC@HM has high safety in vivo,and the blood routine and liver and kidney function of mice are normal after treatment.Conclusion: a new type of nano-platform wrapped by a biomimetic hybrid membrane has been successfully constructed,which can resist tumor through the combination of photothermal therapy,photodynamic therapy,and chemotherapy,and can enhance tumor targeting and prolong the halflife of drugs.reduce drug side effects and increase drug utilization.Such a safe and efficient nano-drug loading platform has potential clinical significance for the treatment of gastric cancer. |
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