| Objective:To observe the intervention effects of different doses of Maren Pills on slow transit constipation(STC)model rats,to explore the"dose-effect" relationship,to verify the efficacy,and to explore colonic aquaporin 3(AQP3)and cystic fibrosis transmembrane Transduction regulatory factors(CFTR),acetylcholine(Ach),vasoactive intestinal peptide(VIP),inflammatory factors and other changes in treatment and their correlations provide a basis for the standardized treatment and medication of STC.Methods:The 48 healthy male Wistar rats were divided into 6 groups according to the random number table method(8 in the blank group,8 in the model control group,and 32 in the drug intervention group).Except for the blank group,the other groups were given loperamide suspension to establish an STC rat model,and the blank group was given the same amount of distilled water by gavage at the same time.The model control group only builds models without intervention,and the remaining 4 groups receive intervention after the model is stabilized.By comparing the general condition of rats in each group,the advancing rate of small intestine charcoal powder,the time of first black stool,the water content of stool,etc.,the therapeutic effects of different doses of Maren Pills on STC were observed.ELISA was used to determine the contents of Ach and VIP in rat serum.Immunohistochemistry was used to observe the distribution and expression of AQP3 and CFTR in colon tissue sections.Real-time fluorescent quantitative PCR was used to detect the mRNA expression levels of AQP3 and CFTR.Results:(1)Thirty days after modeling,compared with group A,rats in groups B,C,D,E,and F had fewer fecal pellets in 24 hours(P<0.01),fecal water content decreased(P<0.01),and the first black stool The time is prolonged(P<0.01).(2)After 7 days of drug intervention,compared with group A,rats in groups C,D,E,and F decreased the fecal water content,charcoal pushing rate,and number of fecal particles in 24 hours(P<0.01);compared with group B,C,D,E,F groups 24h fecal water content,charcoal pushing rate and 24h fecal particle number all increased(P<0.05),D and E groups were better than C and F groups in improving 24h fecal water content(P<0.05),but there was no significant difference in improving the charcoal advancing rate and the number of fecal particles in 24h(P>0.05).(3)After Mosapride and Maren Pills intervened in STC model rats for 7 days,the Ach concentration in serum of rats in groups C,D,E,and F was higher than that in group B,and the VIP concentration was lowered(P<0.05);C The expressions of CFTR mRNA and CFTR protein in the intestinal tissues of rats in the,D,E,and F groups increased,and the D and E groups were more obvious than the C,F groups and the B group(P<0.05)and the D and E groups Compared with group A,the expression decreased,but the difference was not statistically significant(P>0.05);the expression of AQP3 mRNA and AQP3 protein in the intestinal tissues of rats in groups C,D,E,and F showed a downward trend compared with group A,but there was a difference There was no statistical significance(P>0.05)and there was no significant change compared with group B.Conclusion:(1)By regulating the secretion of Ach and VIP in rats,Maren Pills can regulate both intestinal motility and intestinal fluid secretion.This may be one of the mechanisms of Maren Pills in treating constipation.(2)Maren pill has a great influence on the expression level of CFTR in rats,but has little effect on the expression of AQP3.It is possible that Maren pill can increase the secretion of intestinal water fluid,but does not affect the absorption of intestinal water fluid.The effect of "enhancing fluid/moisturizing intestines" treats constipation.(3)The effect of Maren Pills in up-regulating the expression level of CFTR in rats is better than mosapride,and the effect of middle and high doses is better than that of low doses,and there is no significant difference between the middle and high doses. |
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