Clinical Significance Of Changes In Gut Microbiota And Its Metabolites In Kawasaki Disease

Objective Through the analysis of the structure,diversity,metabolites and pathways of gut microbiota in children with Kawasaki disease(KD),the clinical significance of changes in gut microbiota and its metabolites in the course of KD were studied,in order to find the role of gut microbiota in the occurrence and development of KD.Methods A total of 20 children with KD were included in this study.Fecal samples were collected during the acute stage of KD,when the body temperature was normal after treatment,and during the recovery stage.The 16SrDNA gene was detected by high-throughput sequencing for bacteriological classification,and the clinical significance of changes in gut microbiota during the course of KD were analyzed in terms of community structure,α diversity and β diversity.Metabo-profile method was used to compare the changes of gut microbiota metabolites in the course of KD in children by self-matching.Results The a diversity analysis of gut microbiota in children with KD showed that the Chao index of the three groups were 1179.209,1453.137,1565.357,and the Shannon index was 2.06,2.50,1.14.There was no significant difference among the three groups(P>0.05).The βdiversity analysis showed that the gut microbiota of the three groups were similar,and there was no significant difference in the structure of the gut microbiota among the three groups(P>0.05).OTUs cluster analysis showed that Firmicutes,Bacteroides,Proteobacteria and Actinomycetes were the dominant phyla in the three groups,Proteobacteria and Actinomycetes showed a downward trend and then upward trend in the three groups.The metabolites were enriched in linoleic acid metabolic pathway.Conclusion The α-diversity and β-diversity of gut microbiota did not change significantly during the course of KD.Proteobacteria and Actinobacteria showed dynamic changes in the course of KD.Metabolites may be related to linoleic acid metabolism pathway.The changes of gut microbiota and its metabolites may be related to the course of KD;Metabolites are related to linoleic acid metabolic pathway,which may provide a new way to explore the pathogenesis of KD.