Preliminary Study Of Embryonic Stem Cell Mediated Decellularized Kidney Scaffolds Repair And Regeneration

Objectives: To prepare SD rat decellularized kidney scaffolds and detect their relevant characteristics,and to preliminary study the effect of using embryonic stem cells combined with decellularized kidney scaffolds to repair and regenerate part of the kidney damage.Methods: The perfusion channel was obtained by ligating the vascular branches from the abdominal aorta to the renal arteries,and a peristaltic pump was used to perfuse heparin,Triton X-100,SDS and other solutions into the kidney through the abdominal aorta through the channel to prepare the SD rats decellularized kidney scaffolds.By observing the relevant conditions of the prepared decellularized kidney scaffold,including the scaffold in general,tissue staining,microstructure retention and cytokine content,etc.,so as to obtain decellularized kidney scaffolds that suitable for transplantation.The embryonic stem cells were further cultured in vitro,and the embryonic stem cells of appropriate concentration are implanted into the stent through the renal artery and ureter of the decellularized kidney scaffold.Select age-appropriate male SD rats.After anesthesia,take a longitudinal incision of about 2cm in the left ventrolateral region and partially excise the lower pole of the kidney.The control group: the lower part of the decellularized kidney scaffold was used to cover the remnant wound of kidney after excision;Experimental group: the lower part of the decellularized kidney scaffold with rat embryonic stem cells was used to cover the remnant wound of kidney after excision.After the operation,the relevant conditions of kidneys after transplantation were observed at the first week,the second week,and the fourth week,including the use of ultrasound to detect kidney blood flow and the range of decellularized kidney scaffolds,the weight of lyophilized kidney,kidney function and related cytokines.Analyze and compare the results obtained from the experiment.Results: The appearance of the decellularized kidney scaffold prepared by infusion of heparin,Triton X-100,SDS in turn is transparent,its cellular components can be completely removed,the microstructure remains relatively complete,and the measured cytokines are not significant changed compared to ordinary kidney.In the application of the decellularized kidney scaffold to repair some kidney defects,both the control group and the experimental group showed that the kidney parenchyma hyperplasia towards the decellularized kidney scaffold.At the same time,the weight of the kidney tissue increased,the blood flow signal in the kidney increased,and the kidney function recovered,but there was no significant difference between the two groups.The levels of TGF-β and VEGF in the transplanted kidney tissue of the control group and the experimental group increased,but the expressions of TGF-β and VEGF factors in the experimental group was higher than those in the control group,with statistical difference.Conclusions: We can prepare a complete decellularized kidney scaffold by perfusion of the decellularized kidney cell fluid,this scaffold can provide a good growth and differentiation environment for stem cells.Injecting embryonic stem cells into this natural tissue engineering scaffold material,and then transplanting part of this scaffold back into the body to repair the damaged kidney,can guide the damaged kidney to regenerate and be more active,for the future use of decellularized kidney scaffold and embryonic stem cells to regenerate The study of the kidney provides a broader perspective.