| Due to the disorder of purine metabolism or uric acid excretion in the human body,the concentration of blood uric acid is too high,causing a variety of diseases based on hyperuricemia.Research on reducing uric acid production or promoting uric acid excretion is quite important.Xanthine oxidase plays an extremely important role in the process of catalyzing the production of uric acid.Therefore,xanthine oxidase inhibitors can effectively improve hyperuricemia.In the context of the large health industry,looking for natural and safe xanthine oxidase inhibitors is a new trend in research.China has a wealth of food and medicine homologous plant resources,and the safety of use is high.This topic takes food and medicine homologous resources as the research object,selects xanthine oxidase inhibitors from it,and conducts prescription research and extraction process optimization,with a view to The research and development of uric acid-reducing health food lay the foundation and provide scientific basis.In this study,the water extracts of 20 kinds of food and medicine resources were screened,and it was found that the water extracts of the three plants of chrysanthemum,honeysuckle,and lotus leaf were better,and then the combination of the xanthine oxidase inhibitory effect was carried out Proportion research,found the optimal combination ratio,and further carried out relevant experimental verification in mice.On this basis,with the extraction rate as the evaluation index,the response surface method extraction process optimization and antioxidant capacity determination of lotus leaf,honeysuckle and chrysanthemum were carried out respectively.The results of the study showed that the water extracts of lotus leaf,honeysuckle,and chrysanthemum have an IC50 of 19.723 mg / m L,19.347 mg / m L,and 20.280mg/ m L for xanthine oxidase,respectively,all showing good inhibitory activity and showing dose dependence Sex,high-dose effect is most obvious.The three water extracts were tested in vitro according to different combination ratios.It was found that honeysuckle,chrysanthemum and lotus leaf were combined in a ratio of 1: 1: 1,which had the best inhibitory effect on xanthine oxidase at a concentration of 20 mg /m L.When the inhibition rate is 57.6%.Lotus leaf,honeysuckle and chrysanthemum were combined in a 1:1:1 ratio to conduct uric acid lowering experiments in mice.Without adverse effects,it canprovide corresponding reference basis for the research and development and utilization of food and medicine homologous resources health products.Under the optimization of the response surface method,a better extraction process was obtained for the water extracts of three food and medicine resources.The optimized extraction process of lotus leaf water extract is 90 min extraction time,80 ℃ extraction temperature,2 extraction times and 1:30 material-liquid ratio.The optimized extraction process of honeysuckle water extract is 90 min extraction time,90 ℃ extraction temperature,3 extraction times,and 1:40 liquid-to-liquid ratio.The optimized extraction process of chrysanthemum water extract is 90 min extraction time,90 ℃ extraction temperature,2 extraction times,and 1:30 liquid-material ratio.The process method using response surface optimization is highly accurate,and provides a reference for the extraction process for the development of subsequent health foods.In the study of antioxidant capacity,it was found that the ability to scavenge hydroxyl free radicals was honeysuckle> lotus leaf> chrysanthemum,the ability to inhibit DPPH free radicals was chrysanthemum> lotus leaf> honeysuckle,and the ability to inhibit ABTS free radicals was chrysanthemum > Honeysuckle > Lotus leaf. |
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