Screening Of Anti-gastric Ulcer Active Constituents From Ethyl Acetate Part Of Chaenomeles Speciosa (Sweet) Nakai And Preliminary Study On Its Chemical Components

Objective:To investigate the anti-gastric ulcer activity of the ethyl acetate part of Chaenomeles speciosa(Sweet)Nakai and screen its active components,and to study the chemical components of the active components,which lays a foundation for further clarifying the material basis of Chaenomeles speciosa(Sweet)Nakai anti gastric ulcer.Methods:(1)The gastric ulcer model was induced by indomethacin in GES-1.The proliferation rate of GES-1 cells was calculated by MTT method.(2)Guided by the anti-gastric ulcer activity,macroporous adsorption resin can be used to selectively adsorb and desorb the components with similar physical and chemical properties from the solution,and the components with anti-gastric ulcer activity in the ethyl acetate of Chaenomeles speciosa(Sweet)Nakai can be screened and enriched.(3)HPLC method was used to analyze the chemical composition of the active components of anti-gastric ulcer.The characteristic peaks of the active components of anti-gastric ulcer in the ethyl acetate part of Chaenomeles speciosa(Sweet)Nakai were demarcated by self-made reference and commercial reference,and the chemical composition of the active components of anti-gastric ulcer in the ethyl acetate part of Chaenomeles speciosa(Sweet)Nakai was determined.Results:(1)The ethyl acetate part of Chaenomeles speciosa(Sweet)Nakai had good anti-gastric ulcer activity and showed a good dose effect relationship.(2)The results showed that D101 macroporous resin had a better effect on the active fraction of ethyl acetate in Chaenomeles speciosa(Sweet)Nakai,and its active components of anti-gastric ulcer were concentrated in 20%and 40%ethanol elution sections.The yield of D101 macroporous resin was 21.76%as the active component of ethyl acetate in the Chaenomeles speciosa(Sweet)Nakai.(3)There were 12 characteristic peaks in the anti-gastric ulcer active component screened from the ethyl acetate part of Chaenomeles speciosa(Sweet)Nakai,and the corresponding peak is s peak(peak 3).The relative retention time of each characteristic peak and s peak is calculated.The specified values of the relative retention time are 0.547(peak 1),0.577(peak 2),1.000(peak 3),1.446(peak 4),1.492(peak 5),1.736(peak 6),1.988(peak 7),2.155(Peak 8),2.964(peak 9),5.157(peak 10),7.155(peak 11),7.584(peak 12).Compared with the control chromatogram peak,it was identified that 3 peak was Protocatechuic Acid(t _R=6.06min),4 peak was Chlorogenic acid(t _R=8.76min),5 peak was Catechin(t _R=9.04min),8 peak was Epicatechin(t _R=13.06min),9 peak was Rutin(t _R=17.96min),11 peak was Kaempferol(t _R=43.36min),12 peak was hawthorn acid(t _R=45.96 min).Conclusion:In this study,the anti-gastric ulcer active component of ethyl acetate in the Chaenomeles speciosa(Sweet)Nakai was screened,and its HPLC chromatogram was established.The characteristic peaks of the anti-gastric ulcer active component of ethyl acetate in the Chaenomeles speciosa(Sweet)Nakai were demarcated by self-made reference and commercial reference,and the chemical components of the anti-gastric ulcer active component of ethyl acetate in the Chaenomeles speciosa(Sweet)Nakai were identified preliminarily,which laid a foundation for further clarifying the material basis of the Chaenomeles speciosa(Sweet)Nakai in anti-gastric ulcer.