Feasibility Study On The Treatment Of Prostatitis-caused Bladder Dysfunction By P2X3 Receptor Inhibitor PPADS

Objective By establishing the rat model with chronic nonbacterial prostatitis to explore the expression of P2X3 receptors in bladder dysfunction,and to explore the efficacy of PPADS in the treatment of the prostatitis-caused bladder dysfunction.Methods Aseptic carrageenan was injected into the prostate of the rats to establish the model of chronic nonbacterial prostatitis.To explore the changes of bladder function with urodynamics.The expression of P2X3 receptor protein in L4/L5 segment dorsal root ganglion and bladder tissues in model rats was detected by cellular immunofluorescence.Results(1)The rat model of chronic nonbacterial prostatitis was established successfully,The results of HE staining showed that there was no inflammatory reaction in the bladder during the establishment of the rat model of prostatitis.(2)Immune histochemical proves that the expression of P2X3 in bladder and L5-L6 nerves is higher than that of in normal rats,suggesting P2X3 is regulate to the urination dysfunction of bladder and prostate,prostatitis can result in urination dysfunction and overexpression of P2X3.(3)Urology in rats showed that the urodynamic parameters of the experimental group were higher than those of the blank group and control group except bladder volume.(4)After the perfusion treatment with PPADS,there was no significant difference in bladder volume and basal pressure among the three groups,while the pre-urination pressure,maximum urination pressure and urination frequency decreased,with statistically significant differences.Moreover,P2X3 expression in the bladder tissue and l5-l6 nerve tissue of the three groups was lower than that before the perfusion treatment with PPADS.Conclusion It was confirmed by HE staining that the injection of sterile carrageenan into the rat prostatitis could successfully establish the model of non-bacterial prostatitis,and the inflammation was confined to the prostate but did not extend to the bladder.Bladder pressure urodynamics suggests that prostatitis can cause changes in bladder function,mainly due to overactive bladder and increased urethral pressure.Further immunofluorescence confirmed bladder epithelial tissue and L4 to L6 nerve of P2X3 receptor expression,and the expression of prostatic inflammation group content is higher than the rest of the group,indicate prostate inflammatory stimulation can cause bladder epithelial tissue and L4 to L6 nerve increase in P2X3 receptor expression,and after using PPADS bladder epithelial tissue section were nerve and L4 to L6 P2X3 receptor expression is reduced,the bladder to activity decreases and the voiding pressure significantly decreased.So we conclude that the prostate inflammatory stimulation can cause bladder epithelial tissue and L4-L6 P2X3 receptor expression in dorsal root nerve,and P2X3 receptor expression increase factor stimulating basin may be through some nerve bladder voiding dysfunction,such as excessive contraction to use its antagonist PPADS can reduce prostatitis caused by bladder dysfunction.