A Study On The Correlativity Between Tumor Infiltrating Lymphocytes And Clinical Pathology Of Breast Cancer

Background:Tumor-infiltrating lymphocytes(TILs)are considered to play an important role in tumor-related immune responses,and more and more evidence shows that TILs and their subtypes of TILs have great value in breast cancer related research.Methods: The primary invasive breast cancer specimens of 112 patients with complete relevant pathologic information were sliced.HE(hematoxylin-eosin)staining and related CD3,CD4,CD8 immunohistochemistry were performed.The count of CD3,CD4,CD8 tumor infiltrating T-lymphocyte and TILs level had been estimated.Tumor diameter,lymph node metastasis status,HER2,ER,PR,Ki-67,typing of breast cancer and prognosis were studied in correlation,P value less than0.05 was statistically significant.Results: The results of the present study demonstrated that low levels of TILs are associated with hormone receptor–positive,low Ki-67 expression,and the levels of TILs are associated with breast cancer molecular typing.All types of TILs in this study,the counts are positively correlated with the level of TILs.The high CD3+TILs count was correlated with high Ki-67 and HER2 expression.The low count of CD4+ TILs was correlated with hormone receptor–positive.There were differences in the group of luminal A breast cancer and TNBC.Low CD8+ TILs count is associated with low expression of Ki-67 and HER2,positive expression of ER and PR.The high count of CD4+ and CD8+ tumor infiltrating T lymphocyte is significantly associated with no lymph node metastasis.Conclusions: This study found that the levels of TILs and the counts of CD3+,CD4+,CD8+ tumor infiltrating T lymphocyte were related to some clinicopathological elements,of which TILs levels,CD4+ tumor infiltrating T lymphocyte counts were also related to molecular typing.CD4+ and CD8+ tumor infiltrating T lymphocyte counts were significantly correlated with no lymph node metastasis,we considered that the higher the CD4 + and CD8 + tumor infiltrating T lymphocyte counts indicated the better prognosis.