The Effect Of Anti-VEGF Drugs On The Proliferation And Epithelial-mesenchymal Transition Of Human Lens Epithelial Cells

Objective: To explore the effect of the anti-VEGF drug Conbercept on the proliferation and epithelial-mesenchymal transition of human lens epithelial cells(Human Lens epithelial cells,HLECS),and provide experimental evidence and ideas for clinical prevention and treatment of PCO.Methods: The experiment was divided into 5 groups,namely the control group(0 mg/ml)and four different concentrations of Conbercept.The concentrations of Conbercept were(0.1 mg/ml,0.5 mg/ml,1.0 mg/ml,2.0 mg/ml),the cultured human lens epithelial cell line SRA01/04 was treated with different concentrations of Conbercept,the MTT method was used to detect the effect of Conbercept on the survival rate of lens epithelial cells,and flow cytometry For cell cycle and apoptosis,the expression levels of Bax,Bcl-2,E-cadherin,Vimentin,and Slug were detected by Western blot(WB).Results: As the concentration of Conbercept increased,the survival rate of the cells gradually decreased.Compared with the control group,the survival rate of the cells in the0.1 mg/ml group was not statistically significant(P>0.05),the 0.5 mg/ml group,1.0mg/ml group,and 2.0 mg/ml group,the cell survival rate decreased were statistically significant(P<0.05);with the increase of the concentration of Conbercept,the proportion of cells blocked in the G0/G1 phase gradually increased.Compared with the control group,the proportion of cells blocked in G0/G1 phase in the 0.1 mg/ml group and 0.5 mg/ml group was not statistically significant,while in the 1.0 mg/ml group,the increase in the proportion of cells in the G0/G1 phase was statistically significant(P<0.05),and the increase in the proportion of cells in the G0/G1 phase in the 2.0 mg/ml group was significantly different(P<0.01);the rate of cell apoptosis increased with the increase of conbercept concentration gradually increased,and each group was statistically significant compared with the control group(P<0.05);the expression level of apoptosis-promoting protein Bax increased,and the 0.1 mg/ml group was compared with the control group.The ratio was not statistically significant(P>0.05),the 0.5 mg/ml group,1.0 mg/ml group,and 2.0 mg/ml group were statistically significant compared with the control group(P<0.05);The expression level of apoptosis inhibitor protein Bcl-2 gradually decreased,and each group was statistically significant compared with the control group(P<0.05);the expression level of the protein E-cadherin associated with epithelial-mesenchymal transition gradually decreased,and the 0.1 mg/ml group was compared with the control group,it was not statistically significant(P>0.05).The 0.5 mg/ml group and 1.0 mg/ml group were statistically significant compared with the control group(P<0.05),and the 2.0mg/ml group was compared with the control group,There is a significant difference(P<0.01);in the 0.1 mg/ml group and 0.5 mg/ml group,the expression level of Vimentin was lower than that of the control group,but it was not statistically significant(P>0.05).The 1.0 mg/ml group was Compared with the control group,the expression level of Vimentin increased,and there was no statistician significance(P>0.05).Compared with the control group,the expression level of Vimentin increased in the 2.0 mg/ml group,and it was statistically significant(P< 0.05);the expression level of Slug increased compared with the control group.Among them,the 0.1 mg/ml group,0.5 mg/ml group,and 1.0mg/ml group were not statistically significant compared with the control group(P>0.05),The 2.0 mg /ml group was statistically significant compared with the control group(P<0.05).Conclusions: 1.A certain concentration(0.5mg/ml,1.0mg/ml,2.0 mg/ml)of conbercept can reduce the survival rate of lens epithelial cells and inhibit cell proliferation;2.A certain concentration(1.0mg/ml,2.0 mg/ml)of conbercept can block lens epithelial cells in G0/G1 phase;3.conbercept at a certain concentration(0.1mg/ml,0.5mg/ml,1.0mg/ml,2.0mg/ml)can promote the apoptosis of lens epithelial cells;4.High concentration(2.0mg/ml)conbercept can promote epithelial-mesenchymal transition of lens epithelial cells.