Quality Evaluation Of Astragali Radix Praeparata Cum Melle And Hedysari Radix Praeparata Cum Melle Based On Quality Markers Of Traditional Chinese Medicine Control Research

Objective:To study the quality control of Radix Astragali and Radix Hedysari by predicting the quality markers related to their pharmacodynamics.Methods:The quality markers of Radix Astragali and Radix Hedysari were screened by network pharmacology and molecular docking technology.On this basis,the quality control of 15 batches of Radix Astragali and Radix Hedysari was studied by HPLC,cluster analysis,PCA,opls-da and fingerprint.Results:1.Through network pharmacology analysis,23 potential active components of Astragalus membranaceus were found to act on 222 targets in immune deficiency diseases.Among them,pistil isoflavone,formonoside,astragaloside I,astragaloside II,astragaloside III,formononetin,astragaloside IV and isoflavonoside of verbalein could be connected with many targets,and molecular docking results showed that these components were connected with targets Therefore,it may be the potential active component of Astragalus membranaceus on immune deficiency disease.2.Through network pharmacology analysis,16 potential active components of Hedysarum Hedysarum were found to act on 200 targets in immune deficiency diseases.Among them,formonoside,vanillic acid,calycosin,formononetin and calycosin could connect with more targets,and molecular docking results showed that these components had good binding activity with targets,so Hedysarum Hedysarum Hedysarum Hedysarum might play an important role It is a potential active ingredient in immune deficiency disease.3.HPLC-UV,HPLC-ELSD and HPLC-ELSD were established for the determination of eight components in fried Radix Astragali and five components in fried Radix HedysariHPLC-UV:HC-C₁₈column(Agilent company,model:Agilent 4.6 mm×250 mm,The mobile phase consisted of acetonitrile（A）-0.2%formic acid aqueous solution（B）,gradient elution:0～5 min,5%～13%A;5～10 min,13%～21%A;10～23 min,21%～37%A;23～33 min,37%～53%A;33～43 min,53%～69%A;43～45 min,69%～100%A;volume flow rate:1 m L·min^-1;UV:detection wavelength:260 nm;column temperature:30℃;injection volume:5μL.HPLC-ELSD:HC-C₁₈column（Agilent company,model:Agilent 4.6 mm×250mm,5 um）;mobile phase:acetonitrile（A）-water（B）,gradient elution:0～5 min,5%～13%A;5～10 min,13%～21%A;10～23 min,21%～37%A;23～33 min,37%～53%A;33～43 min,53%～69%A;43～50 min,69%-100%A;volume flow rate:1 m L·min^-1;ELSD:atomization temperature:30℃;drift tube temperature:105℃;nitrogen flow rate:2.5 L·min^-1.Methods:HPLC was performed on a HC-C₁₈column(Agilent 4.6mm×250mm,five The mobile phase was acetonitrile（A）-0.01%phosphoric acid aqueous solution（B）,gradient elution,0～5 min,2%～5%A;5～20 min,5%～16%A;20～30 min,16%～23%A;30～35 min,23%～25%A;35～40 min,25%～33%A;40～45 min,33%～36%A;45～60 min,36%-50%A;60～70 min,50%-75%A;70～75 min,75%～75%A,the volume flow rate was 1 m L·min^-1;the detection wavelength was 254 nm;The column temperature was 30℃.4.The test items of Radix Astragali and Radix Hedysari were in accordance with the Pharmacopoeia;A method for the determination of total polysaccharides and total flavonoids in Radix Astragali and Radix Hedysari was established.Meanwhile,a method for the determination of 8 components in Radix Astragali and 5 components in Radix Hedysari was established.5.Cluster analysis and principal component analysis divided 15 batches of roasted Radix Astragali into 3 groups,and opls-da analysis selected 6 different quality markers,which were calycosin,formononetin,calycosin,astragaloside I,astragaloside III and astragaloside IV;cluster analysis and principal component analysis divided 15 batches of roasted Radix Astragali into 3 groups,and opls-da analysis selected 4 different quality markers The markers were formononetin,formonoside,vanillic acid and calycosin.Conclusions1.Based on the quality markers of traditional Chinese medicine,from the effectiveness of components,through molecular docking and network pharmacology,it can provide more convenient and effective research methods and paths for the prediction and selection of quality markers.2.Based on the basic characteristics of quality markers of traditional Chinese medicine,the quality markers of Astragalus membranaceus were preliminarily determined to be calycosin,formonoside,astragaloside I,astragaloside II,astragaloside III,formononetin,astragaloside IV and calycosin;The quality markers of Hedysari are formononetin,vanillic acid,calycosin,formononetin and calycosin.3.The quality standards of processed Radix Astragali and Radix Hedysari based on the quality markers of traditional Chinese medicine are stable and controllable,which can be used for the quality evaluation.