Analysis Of Intestinal Microbiome In Neonatal Pneumonia

Part 1 Analysis of short-chain fatty acids,bile acids and ammonia in the feces of neonates with pneumonia Objective:To study the levels and changes of short-chain fatty acids,bile acids and ammonia in the feces of neonates with pneumonia.Methods:A total of 29 children diagnosed with neonatal pneumonia in Shanghai children’s hospital were selected,including 16 males and 13 females,with a median daily age of 18 days.Meanwhile,29 health newborns,including 17 males and 12 females,with a median daily age of 18 days were included.The fecal samples of neonate with pneumonia were collected at the time of admission and the fecal samples of health neonate were collected at the time of physical examination.Short-chain fatty acids in feces were quantitatively determined by gas chromatography.Bile acids and ammonia were determined by biochemical enzyme method.Results:The concentrations of acetic acid,propionic acid,butyric acid,valeric acid,isobutyric acid and bile acid in the newborns with pneumonia were higher and significantly different from those in the normal group(P<0.05).But there was no significant difference in the concentrations of isovaleric acid and ammonia(P > 0.05).Conclusion:The changes of faecal short-chain fatty acids and bile acids in neonates with pneumonia reflect the changes of intestinal microbiota composition,which provides research basis for probiotics to play an auxiliary role in the treatment of neonatal pneumonia.Part 2 16 S rDNA analysis of intestinal microbiota in neonatal pneumonia Objective:To study the difference of intestinal microbiota structure and composition between neonate pneumonia and health neonates.Methods:11 cases of neonatal pneumonia were diagnosed in Shanghai Children’s Hospital,including 5 males and 6 females,with a median age of 21 days.10 cases of health newborns,including 6 males and 4 females,with a median age of 19 days were included in current study.The fecal samples of neonate with pneumonia were collected at the time of admission and the fecal samples of health neonate were collected at the time of physical examination.Paired end 16 s rDNA sequencing was carried out using Illumina miniseq platform,and the results were analyzed using Bioinformatics software.Results:There was no significant difference in α-diversity inside the two groups(P > 0.05),and there was significant difference in β-diversity between the two groups(P<0.05).In the pneumonia group,the dominant phylum was Proteobacteria;in the health group,the dominant phylum was Firmicutes.The relative abundance of Enterococcus and Bifidobacterium in the pneumonia group was significantly higher than that in the health group(P<0.05).In contrast,Lactococcus was significantly higher in health group(P<0.05).Conclusion:The inflammatory status after neonatal pneumonia may have an effect on the composition and structure of intestinal microbiota,resulting in the decline of microbiota diversity and dysbiosis of the intestinal microbiota.