Glaucocalyxin A Induces Apoptosis And Autophagy In Tongue Squamous Cell Carcinoma Cells By Regulating ROS

Background:Tongue squamous cell carcinoma（TSCC）is the most common highly invasive oral cancer with high recurrence rate and poor prognosis,which causes great pain to patients.GLA is a diterpenoid compound with antibacterial and anticancer biological characteristics.It has a wide range of effects and has anticancer effect in a variety of malignant tumors.However,the anticancer effect and potential mechanism of GLA in TSCC have not been reported.Objectives:This experiment explores the effect of GLA on the proliferation of TSCC cells,and explores the possible mechanism of GLA on the treatment of TSCC from the aspects of apoptosis,autophagy and reactive oxygen species,and strives to find an effective and low toxicity drugs to treatment TSCC.Methods:The activity of CAL27,Tca8113 and normal cells（HGE）was detected by CCK-8 method.In CAL27 and Tca8113 cells,the proliferation ability was detected by plate cloning experiment.DAPI staining and Lyso-Tracker Red staining were used to detect changes in nuclear morphology and changes in autophagy.Flow cytometry was used to detect changes in cell apoptosis.CM-H₂DCFDA fluorescent probe is used to detect the level of reactive oxygen species in cells.Western blot is used to detect the expression levels of apoptosis and autophagy-related proteins.Results:GLA significantly reduces the activity of TSCC cells and inhibits their proliferation ability in a dose-and time-dependent manner.But it has no obvious effect on HGE cells.GLA induces TSCC cell apoptosis,autophagy and ROS production in a dose-and time-dependent manner.In addition,GLA inhibits the activity of TSCC cells by inducing the production of intracellular ROS.Finally,GLA triggers ROS-dependent apoptosis and autophagy in TSCC cells.Conclusion:GLA can inhibit cell viability,trigger apoptosis and autophagy by inducing intracellular ROS production.