Effects Of Long Non-Coding RNA NCK1-AS1 In Esophageal Squamous Cell Carcinoma

Objective:There is growing evidence that long non-coding RNAs(lncRNAs)serve vital functions in cancer progression,and the distinct overexpression of NCK1-AS1 was frequently reported in many tumors,this lncRNA affects the ability of proliferation and metastasis.However,few studies have been conducted on NCK1-AS1 in esophageal squamous cell carcinoma(ESCC),and its effect in ESCC remains unclear.The purpose of this study was to investigate the expression and the effect of lncRNA NCK1-AS1 on proliferation,migration,invasion,and apoptosis of the esophageal squamous cell carcinoma cell.Method:1.ESCC gene expression dataset GSE17351 was obtained from the Gene Expression Omnibus(GEO)database.We used the gene annotation platform to identify differentially expressed genes.The R software was used to process the gene expression data and identify the differentially expressed genes.GEPIA,a database for cancer-related gene expression analysis and interactive analysis was used to verify differential lncRNA and gene expression.2.qRT-PCR was used to detect the expression levels of NCK1-AS1 in normal esophageal epithelial cells HET-1A and ESCC cell line EC109,EC9706,TE-10,and KYSE-150.NCK1-AS1 was inhibited by constructing specific si RNA and transfected into ESCC cells.qRT-PCR was used to detect the effect of si-NCK1-AS1-1 and si-NCK1-AS1-2 on the expression of NCK1-AS1 in cell line EC109 and EC9706 and the interference efficiency.3.Cell counting kit-8 assay,transwell assay,scratch test,and flow cytometry were respectively used to detect the effects of si-NCK1-AS1-1 and si-NCK1-AS1-2on the proliferation,migration,invasion,and apoptosis of cell line EC109 and EC9706.Results:1.Bioinformatics identified NCK1-AS1 as a differentially expressed gene,which was further confirmed in the GEPIA database with abnormally high expression of NCK1-AS1 in ESCC.2.The results of qRT-PCR showed that NCK1-AS1 was significantly overexpressed in four ESCC cells compared with cell line HET-1A,and si-NCK1-AS1-1 and si-NCK1-AS1-2 had significant inhibitory effects on NCK1-AS1 expression in cell line EC109 and EC9706(silenced efficiency > 70%).3.The results of the cell counting kit-8 assay,transwell assay,scratch test,and flow cytometry showed NCK1-AS1 knockdown inhibited the proliferation,invasion,migration,but enhanced apoptosis of ESCC cells.Conclusion:LncRNA NCK1-AS1 was abnormally highly expressed in ESCC cells,lncRNA NCK1-AS1 knock-down inhibited the proliferation,invasion,migration,but enhanced apoptosis of ESCC cells...