Effects Of Chaidamu Black Fruit Lycium Barbarum Anthocyanin On Senescence,p53 And P21 Of Human Skin Fibroblasts Cultured By UVB Radiation In Vitro

Objective 1.To establish UVB radiation-induced premature senescence model of HSFs cells;2.To study the effect of anthocyanins on the premature aging process of UVB radiation damaged HSFs,and to explore the mechanism of inhibiting the premature aging process.Methods Human skin fibroblasts from the third to the fifth generations in the logarithmic growth phase were divided into control group,UVB irradiation group,LBP anthocyanin 100ug/ml+ UVB irradiation group,LBP anthocyanin 500ug/ml+UVB irradiation group,LBP anthocyanin 1000ug/ml+ UVB irradiation group.Except for the control group,the other groups were treated with light and/or drugs as described above.After 24 hours of anthocyanin intervention,the cells were treated with light for 3 consecutive days.After the completion of light treatment,the cells were cultured for 72 hours.(1)Aged cells were stained with SA-β-Gal,and the staining was confirmed by optical microscope and the number of positive Aged cells was calculated.(2)Real-time PCR was used to calculate the expression levels of age-related genes p53 and p21 in each group.Results 1.The UVB radiation-induced premature aging model of HSFS was successfully established;2.Anthocyanins of Lycumbar barum can reduce the premature senescence of HSFS induced by UVB radiation.Compared with the blank group,the number of senescent HSF cells and the positive rate of SA-β-gal staining were significantly increased in the UVB radiation group(P < 0.01).Compared with the UVB irradiation group,the positive rate of SA-β-gal staining in the experimental groups treated with anthocyanin decreased(P< 0.05),and the positive rate decreased more obviously with the increase of drug concentration.3.Anthocyanin can down-regulate the expression of aging related genes p53 and p21 after UVB irradiation of HSFS.Compared with the UVB irradiation group,the expression of aging genes p53 and p21 in different concentrations of anthocyanin in the treatment groups decreased with the increase of drug concentration(P< 0.05).Conclusion Anthocyanins can inhibit the premature aging process of HSFS induced by UVB radiation and have a photoprotective effect.The molecular mechanism may be re-duce the UV damage of HSFS by downregulating the expression levels of p53 and p21.