Mitochondrial Transcription Factor A In Ischemic Stroke Preliminary Study On Expression In Different Tissues

Objective:To study the expression levels of mitochondrial transcription factor A(TFAM)in brain,myocardium and skeletal muscle at different times of ischemic stroke,and to provide clues for the study of the effects of TFAM on the physiological and pathological processes of brain,myocardium and skeletal muscle in ischemic stroke.Methods: Forty-five healthy adult male SD rats were enrolled randomly.The rats were divided into three groups according to different experiment contents,with 15 rats in each group.The Control group(Control)was not treated and fed normally.Sham operation group(SHAM)was used to treat the same middle cerebral artery occlusion(MCAO)group,but no thrombus was inserted.The middle cerebral artery was blocked by the right common carotid artery by modified thread embolism in MCAO group.The SHAM group and the MCAO group were randomly divided into three time points: 2h,4h and 12 h.Blood samples of the Sham group and the MCAO group were collected preoperatively and postoperatively at 2h,12 h for analysis of the number of blood cells and related indexes.Zea Longa positive score was used to evaluate the central nervous function of the awake rats.Niss staining was used to accurately observe the changes of neuronal cells and Nissi staining in the cortical region of brain tissue.Real-time fluorescence quantitative PCR(q PCR)was used to detect the changes of m RNA levels in brain,myocardium and skeletal muscle.Results:(1)Assessment of neurological function: The neurological symptoms in the MCAO group were significantly deficient with the duration of ischemic stroke increasing.(2)Niss staining showed that the number of nissonite bodies in the cerebral cortex decreased significantly in the MCAO group,and the number of neurons decreased with the extension of infarct duration.(3)1.The q PCR results of different ischemia time in the same tissue of MCAO group showed that:(1)TFAM gene in brain tissue was decreased at 2h compared with the control group,and then increased,P < 0.05.(2)The expression of TFAM gene in myocardial tissue at2 h was higher than that in the control group,and the levels of MCAO at 4h and 12 h were lower than those in the control group,and the expression at 12 h was lower than that in the control group(P < 0.05).(3)The TFAM gene in the MCAO group at 2h and 4h was significantly higher than that in the control group,and higher than that in the 12 h group(P < 0.05).2.The q PCR results of different tissues at the same ischemia time in MCAO model showed that the m RNA levels of MCAO2 h and4h TFAM in skeletal muscle > myocardium > brain,P < 0.05,except for the requirement that 2h myocardium was higher than that in brain,and the difference was not significant.The level of TFAM m RNA in the brain of MCAO12 h rats was lower than that in the myocardium.3.The expression of TFAM was also significantly changed in the Sham group.Conclusion: It is found that TFAM is highly sensitive to external stimuli.At different times of acute ischemic stroke,the expression levels of different tissues are different,and TFAM may induce ischemia and hypoxia adaptation of brain,myocardium and skeletal muscle in ischemic stroke.