Effects Of Atorvastatin On Inflammatory Factors And Seizures In Lithium-pilocarpine Rat Model Of Epilepsy

BackgroundEpilepsy is a neurological electrical-clinical syndrome caused by multiple etiologies,and the pathogenesis is not yet fully understood.Inflammatory factors such as interleukin-1β(IL-1β),interleukin-6(IL-6),and tumor necrosis factor-α(TNF-α)play a biological role in many physiological and even pathological activities of the body effect.In addition to regulating lipids,statins also have neuroprotective effects such as anti-oxidative stress and anti-inflammatory.The previous study of our group found that statins also have anti-epileptic effects.However,the correlation between statin therapy and the levels of IL-1β,IL-6 and TNF-α and the effects on the various stages of the rat epilepsy model are still unclear.therefore,This study uses an animal model of epilepsy induced by lithium-pilocarpine,To explore the effect of atorvastatin on epileptic seizures and analyze its related possible mechanisms.ObjectiveTo study the behavioral effects of Atorvastatin(ATV)on SD rats with lithium-pilocarpine(LI-PILO)epilepsy model.and the correlation with the levels of inflammatory factors(IL-1β,IL-6,TNF-α),To explore whether ATV has a neuroprotective effect on the epilepsy model induced by LI-PILO and analyze its related possible mechanisms.Methods1.The epilepsy model was induced by intraperitoneal injection of LI-PILO,and a24-hour video surveillance system was used to observe and record behavioral changes.that Includes the scores of the degree of epilepsy,the incubation time and the incubation period,and the number of spontaneous seizures of SD rats in the experimental group(10mg/kg ATV group and 50mg/kg ATV group)and the control group(saline group)at different time points in the acute phase(10min,20 min,30min,40 min,50min).2.The epilepsy model was induced by intraperitoneal injection of LI-PILO,and the ELISA method were used to detected the levels of serum IL-1β,IL-6,TNF-α and other inflammatory factors in the chronic phase of SD rats in the ATV group and the control group.3.The epilepsy model was induced by intraperitoneal injection of LI-PILO,and the Nissl staining method was used to detect neuronal damage in the chronic phase of SD rats in the ATV group and the control group.4.Use SPSS 23.0 statistical software to statistically analyze the data,t-test to evaluate the significance between the two groups;One-way ANOVA and Bonferroni post-test to compare the differences between the groups.Results1.In the acute phase of the LI-PILO-induced epilepsy model,compared with the control group,the level of seizures in the 10 mg/kg and 50 mg/kg ATV groups decreased significantly at different time points(20min、30min、40min),and the difference was statistically significant(P<0.001 和 P<0.001).In the acute phase,compared with the control group,the 10mg/kg and 50mg/kg ATV groups can significantly prolong the incubation time of seizures,and the difference is statistically significant(P<0.05 and P<0.01).In the incubation period,compared with the control group,the 10mg/kg and 50mg/kg ATV groups can prolong the incubation period of seizures,and the difference is statistically significant(P<0.05 and P<0.05).In the chronic phase,compared with the control group,the10mg/kg and 50mg/kg ATV groups can reduce the onset of spontaneous epilepsy,and the difference is statistically significant(P<0.05 and P<0.001).2.In the chronic phase of the LI-PILO-induced epilepsy model,compared with the control group,the IL-1β,IL-6 and TNF-α inflammatory factors in the 10mg/kg ATV group and the 50mg/kg ATV experimental group were significantly lower than those in the control group.and the difference is statistically significant(P<0.05 and P<0.001).3.In the chronic phase of the LI-PILO-induced epilepsy model,compared with the control group,the 10mg/kg and 50mg/kg ATV experimental groups had more neurons survived,and the difference was statistically significant(P<0.01 and P<0.001).ConclusionAtorvastatin may play a neuroprotective effect by inhibiting the expression of inflammatory factors to reduce the epileptic seizures of SD rats in the LI-PILO-induced epilepsy model.