Expression Of MiR-369-3p In Human Hepatocellular Carcinoma And Its Experiment Study For Regulating Of The Proliferation And Apoptosis Of Hepatocellular Carcinoma Cells

BackgroundEarly clinical symptoms of hepatocellular carcinoma are relatively hidden,so most patients have lost the best opportunity for treatment after diagnosis.Currently,intervention,radiotherapy and chemotherapy are usually used to treat patients with advanced hepatocellular carcinoma in clinical practice,but the 5-year survival rate of patients is relatively low due to the large adverse reactions and poor efficacy of patients^[1-2].Studies have shown that miRNAs（micro RNAs）can effectively participate in the occurrence and development of liver cancer by directly regulating the expression of target genes^[3-4].This study mainly investigated the regulatory effect of miR-369-3p on the proliferation and apoptosis of human hepatoma cells by affecting the expression of cyclin D1 protein（cell cycle active protein 1）,P21 protein,Bcl-2 protein（B-lymphocytoma-2 apoptotic protein）,and Bax protein（B-lymphocytoma-2 related protein）.It provides a new idea for clinical diagnosis and treatment.ObjectiveTo investigate the expression of miR-369-3p in hepatocellular carcinoma and its regulatory effects on the proliferation and apoptosis of human hepatocellular carcinoma by affecting the expression of cyclin D1,P21,Bcl-2 and Bax proteins.MethodsIn this study,qRT-PCR（real-time quantitative polymerase chain reaction）was used to detect the expression of miR-369-3p in hepatocellular carcinomar tissues and adjacent tissues respectively.MHCC97H hepatocellular carcinoma cells were cultured in vitro and transfected into MHCC97H hepatocellular carcinoma cells with miR-369-3p mimics and miR-NC（miRNA negative control）using the active lipid acid receptor method.MHCC97H hepatocellular carcinoma cells were transfected with MTT（Methylthiazolyltetrazolium）method to detect hepatocellular carcinoma cell proliferation;The apoptosis of hepatocellular carcinoma cells was detected by flow cytometry.The expressions of Cyclin D1 protein,P21 protein,Bcl-2 protein and Bax protein were detected by Western blot.Results1.The expression of miR-369-3p in hepatocellular carcinoma tissues was significantly lower than that in adjacent tissues（P<0.05）;2.In vitro cell experiments,compared with the miR-NC group,the expression level of Cyclin D1 protein was significantly decreased in miR-369-3p group,while the expression level of P21 protein was significantly increased,and the cell viability was significantly decreased（P<0.05）;The expression level of Bcl-2 protein was significantly decreased,the expression level of Bax protein was significantly increased,and the apoptosis rate was significantly increased（P<0.05）,suggesting that the overexpression of miR-369-3p could inhibit the proliferation of hepatocellular carcinoma cells and promote the apoptosis of hepatocellular carcinoma cells.Conclusion1.The expression of miR-369-3p was low in hepatocellular carcinoma tissues and high in adjacent tissues.2.MiR-369-3p can inhibit the proliferation of HCC cells by negatively regulating the expression of Cyclin D1 protein and positively regulating the expression of P21 protein;miR-369-3p can promote the apoptosis of hepatocellular carcinoma cells by negatively regulating the expression of Bcl-2 protein and positively regulating the expression of Bax protein.