Effects And Mechanism Of Silybin On Airway Inflammation And Mucin 5ac Hypersecretion In Asthmatic Mice

Objective: To observe the effect of silibinin intervention on airway inflammation and airway Muc5 ac expression in asthmatic mice,and to explore its possible mechanism of action.Methods: 40 SPF female BALB/c mice aged 6-8 weeks were randomly divided into normal group(NS group),asthma group(AS group),Dexamethasone group(DEX group),silybin 40 mg/kg Group(Silybin40 group),silybin 80mg/kg group(Silybin80 group),each group has 8 animals.The AS group was given intraperitoneal injection of 20% egg protein aluminum hydroxide gel solution combined with subcutaneous injection on the 1st and13 th day of the experiment.From the 19 th to the 23 rd day,the 10% ovalbumin solution was used for 30 minutes each time and aerosolized excitation once a day.In the NS group,phosphate buffer was used for sensitization,and 0.9% sodium chloride solution was used for challenge.DEX group,Silybin 40 group,and Silybin 80 group were injected intraperitoneally with Dexamethasone 2mg/kg,silybin 40mg/kg and silybin 80mg/kg 30 minutes before each atomization,and the rest of the treatment was the same as AS Group,all experimental mice were sacrificed 24 hours after the last challenge.Collect mouse BALF for total cell count and white blood cell classification count.ELISA detects interleukin-6(IL-6)and tumor necrosis factor α(TNF-α)in BALF.The pathological changes of lung tissues were observed by HE staining.AB-PAS observe the expression of mucin.Masson staining showed fibrous deposition,and immunohistochemical detection of ERK,p ERK,SP1,and Muc5 ac protein expression levels.Results: Total number of mouse BALF cells,percentage of eosinophils,IL-6,TNF-α,inflammatory cell infiltration scores around the airway,airway mucus positive relative staining area,airway wall thickness,airway collagen fiber deposition area p ERK,SP1,and Muc5 ac protein expression in lung tissue: AS group was higher than NS group,DEX,Silybin40,Silybin80 group was lower than AS group,Silybin40 group was higher than Silybin80 group,the differences between the above groups were statistically significant(p<0.05).The expression of ERK protein in lung tissue: There was no statistically significant difference among the NS group,AS group,DEX group,Silybin40 group,and Silybin80 groups(p>0.05).Conclusion: Silybin inhibits the ERK/SP1 signaling pathway,reduces the expression of inflammatory cytokines and airway Muc5 ac secretion,and reduces airway inflammation and airway remodeling in asthmatic mice.This effect is concentration-dependent.Silibinin may become a potential treatment for asthma.