| Objective To explore the expression of prostate transmembrane protein,androgen induced 1,PMEPA1 in hepatocellular carcinoma tissues,and to analyze its relationship with the clinicopathological characteristics of hepatocellular carcinoma;observe the effect of silencing PMEPA1 on liver cancer The effect of cell HepG2 proliferation and migration.Methods The UALCAN database(http://ualcan.path.uab.edu/)was used to analyze the expression differences of PMEPA1 in hepatocellular carcinoma tissues and adjacent non-tumor tissues in the TCGA database;112 cases of hepatocellular carcinoma paraffin-embedded specimens were collected As the experimental group,another 86 non-tumor liver tissue paraffin-embedded specimens of adjacent cancer were selected as the control group,and the expression of PMEPA1 in hepatocellular carcinoma and adjacent non-tumor liver tissues was detected by the immunohistochemical En Vision method.The Pearson χ2 test method was used Analyze the correlation between PMEPA1 expression in hepatocellular carcinoma tissues and clinicopathological parameters of liver cancer patients;q RT-PCR method to detect the m RNA level of PMEPA1 in hepatocellular carcinoma HepG2;Western blot method to detect PMEPA1 protein in hepatocellular carcinoma HepG2 Use si RNA to interfere with the expression of PMEPA1 in liver cancer cells HepG2;use MTT and Transwell methods to detect the proliferation and migration of silenced PMEPA1 and non-silent PMEPA1 liver cancer cells HepG2.Result(1)The PMEPA1 expression in 50 normal liver tissues and 371 liver cancer tissues was retrieved through the UALCAN database,and the median value of PMEPA1 expression in liver cancer tissues(median 1.448)was significantly higher than that of normal liver tissues(median 1.403),the difference was statistically significant(P <0.01).(2)The results of immunohistochemistry statistics showed that the expression of PMEPA1 protein in liver cancer tissues was significantly higher than that in normal liver tissues,and the difference was statistically significant(χ2=84.42,P <0.0001).(3)The expression of PMEPA1 protein in hepatocellular carcinoma tissue is closely related to tumor diameter(χ2=4.3698,P=0.0366)and lymph node metastasis(χ2=4.4069,P=0.0358),but it is closely related to gender(χ2=0.0872,P= 0.7678),age(χ2=0.0616,P=0.8040),AFP level(χ2=1.0531,P=0.3048),vascular infiltration(χ2=1.1221,P=0.2895),EdmondsonHistological classification(χ2=1.4014,P=0.2365)and TNM staging(χ2=1.0863,P=0.2973)had no significant correlation.(4)The qRT-PCR test results showed that compared with the control group(NC group),the m RNA expression level in the experimental group transfected with si PMEPA1 was significantly down-regulated,and the difference was statistically significant(P <0.01);Western blot test results Show: Compared with the control group(NC group),the protein expression in the experimental group transfected with si PMEPA1 was significantly down-regulated,and the difference was statistically significant(P <0.01).(5)The results of MTT experiment and Transwell experiment showed that compared with the control group,the cell proliferation of HepG2 cells transfected with si PMEPA1 showed a downward trend,and the migration ability was obviously inhibited.The difference was statistically significant(P <0.01).Conclusion(1)PMEPA1 is highly expressed in hepatocellular carcinoma tissue compared with normal liver tissue,and the expression of PMEPA1 is significantly correlated with tumor diameter and lymph node metastasis,suggesting that PMEPA1 is hepatocellular carcinoma Potential molecular markers.(2)PMEPA1 has a promoting effect on the proliferation and migration of liver cancer cells HepG2. |
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