Study On Characteristics And Influencing Factors Of Vaginal Microbiome In Pregnant Women With Gestational Diabetes Mellitus

Objective: To investigate the difference and influencing factors of VBM between gestational diabetes mellitus and non-gestational diabetes mellitusMethods: Selection in anhui medical university affiliated hospital of anqing 2018.1.1-2020.1.31 accept produce sieve single pregnant women queue baseline samples,met inclusion exclusion criteria of gestational diabetes,123 pregnant women group,selected by systematic sampling ratio 1:4 the gestational diabetes,pregnant women,390 controls were included in the object 484,excluding missing or vaginal specimen is unqualified,baseline within 48 hours of sex,baseline report key information does not accord with follow-up records 49,eventually into the object of study,435.Basic information and vaginal secretions samples of all subjects were collected,and vaginal microbiome related data were collected by 16 S rRNA sequencing,and biological information analysis was conducted,as well as relevant statistical analysis.Vaginal microbiome relevant data including the alpha diversity index(Shannon index and observed the number of species),CST classification(mainly divided into lactobacillus dominated and lactobacillus dominated)and bacteria of the genus relative abundance,the basic information of the comparison of the experimental group and control group,vaginal microbiome of the difference of the related data as well as the line of single factor and multiple factors analysis and comparison between groups.Results: Except for age,contraception and pre-pregnancy BMI,there was almost no difference between GDM group and non-GDM group in general(P<0.1),and there was statistical significance in pre-pregnancy BMI between GDM group and non-GDM group(P< 0.001).There was significant difference between LD group and NLD group.GDM group main species of Prevotella Megasphaera,Bifidobacterium,Finegoldia,Anaerococcus.The GDM group main species for Grdnerella,Atopobium,Dialister,Ureaplas-ma;Pregnant women under different general data CST no significant difference,there are differences between the different GDM group CST(P<0.05),age between 25 to 34 years old,the pregnant woman of shannon index(shannon index)is higher than other age groups of pregnant women(P<0.05),the first pregnancy pregnant women shannon index is higher than the first pregnancy pregnant women(P<0.05),the vagina washing habits than normal pregnant women with vaginal washing habits observed that the number of species in a pregnant woman(observer species)high(P <0.05),The observer species of pregnant women with BMI≥24.0 were higher than those of the other two species(P<0.05).The observer species(P<0.05)and Shannon index(P<0.05)of the GDM group were higher than those of the non-GDM group.Gardnerella abundance of non-GDM in the second trimester was higher than that of GDM,and the difference was statistically significant(P<0.05).For pregnant women who did not use contraception,condoms,contraceptives and IUD were all protective factors of GDM(OR<1,P<0.05).Compared with pregnant women with BMI <18.5,those with BMI between 18.5-23.9 and ≥24.0 were risk factors for GDM(OR>1,P<0.05).Compared with NLD group,the risk of developing GDM was lower in LD group(OR<1,P<0.05);GDM increases observer species(Beta>0,P<0.05)and Shannon index(Beta>0,P<0.05).The Shannon index of first-time pregnant women is higher than that of nonfirst-time pregnant women(Beta>0,P<0.05),and the Shannon index increases with the age of pregnant women(Beta>0,P<0.05).Gestational age was the most important factor affecting the genus,and Dialister,Streptococcus,Finegoladia and Anaerococcus significantly negative correlation.Conclusion: The diversity of GDM was higher than that of non-GDM,and the abundance of GDM was related to gestational age.BMI before pregnancy,whether or not contraception and whether lactobacilli are dominant are related to the occurrence and development of GDM in pregnant women.