The Correlation Between The Change Of Microglia/Macrophage Polarization Type And Tumor Biological Characteristics In Recurrent Glioma

Objective:By detecting the expression changes of the two phenotypes of M1 and M2 and the recurrence time interval of glioma-associated microglia and macrophages(glioma-associated microglia and macrophages GAMs)in the same patient’s primary and secondary gliomas,Ki-67,pathological WHO grade changes and other tumor biological characteristics changes to explore the trend of changes in microglia/macrophage polarization types in recurrent gliomas.Methods:From 2013 to 2018,15 patients who were pathologically diagnosed as glioma and underwent primary and secondary surgical treatments,then received regular postoperative radiotherapy and chemotherapy in our hospital were selected.A total of30 specimens were paraffin sectioned.According to the number of operations,the specimens were divided into the first operation group(n=15)and the second operation group(n=15).The marker of M1 phenotype was inducible nitric oxide synthase(i NOS),and the marker of M2 phenotype was CD163.Iunohistochemistry was used to detect the percentage of positive cells for each marker in the two groups.Results:The expression of M2 phenotype in the second operation group of the same patient was higher than that in the first operation group(P<0.05);there was no significant difference between the M1 phenotype of the second operation group and the M1 phenotype of the first operation group(P= 0.63).The Pearson correlation coefficient of the overall M1 phenotype(n=30)and M2 phenotype(n=30)was 0.518,P=0.003,and the two were moderately positively correlated.There was no correlation between the overall M2 phenotype and Ki67 expression(P=0.804),there is no correlation between the change of M2 phenotype in the second operation and the interval between operations(P=0.53).Conclusion:The expression level of M2 phenotype in the second recurrence group specimens was significantly higher than that of the initial group specimens,that is,the expression level of M2 phenotype in recurrent gliomas was significantly increased,while the change of M1 phenotype did not change significantly.This expression level change There is no obvious linear relationship with Ki67,recurrence interval,and WHO classification.The deep relationship needs to be further explored.