Study On The Relationship Between DPP4 Gene Polymorphism And Blood Lipids And Type 2 Diabetes With Coronary Heart Disease

Background: In recent years,with the development of Genome-wide association analysis(GWAS),it has been discovered that some gene single nucleotide polymorphisms(SNPs)are associated with the risk of atherosclerosis in the population.The occurrence of atherosclerotic diseases is determined by both environmental factors and genetic factors,and most of them show complex inheritance patterns.Dipeptidyl peptidase-4(DPP4)plays an important role in atherosclerotic disease.Current research shows that the main pathological mechanism of coronary heart disease is atherosclerosis,and type 2diabetes mellius(T2DM)is coronary heart disease(CAD)The main risk factors for longterm complications and death of T2DM are cardiovascular diseases.Therefore,the study of DPP4 gene polymorphism is helpful to further understand the role of DPP4 in atherosclerosis,and is useful for the development of DPP4 inhibitors and other related Drugs have important guiding significance.Objective: Explore the impact of SNPs at 3 sites in the DPP4 gene(rs3788979、rs4664443、rs13015258)on blood lipid levels,and its relationship on T2DM with CAD.Methods: A collection of 274 patients in the Department of Cardiology,Dongfeng General Hospital Affiliated to Hubei Medical College from January 2019 to December2019,were divided into 152 cases in the control group(no T2DM and CAD)and 122 cases in the case group(T2DM combined with CAD).We previously investigated the differences in the genotype and allele distribution of SNPs at 3 loci(rs3788979、rs4664443、rs13015258)in the DPP4 gene between the two groups,by adjusting for age,gender,and body mass index(BMI)and other confounding factors which were analyzed by logistic regression to study the relationship between DPP4 gene polymorphism and the risk of T2DM with CAD.Results: 1.There is a statistically significant difference in blood lipid levels between the case group and the control group.The level of apolipoprotein B/apolipoprotein A1(ApoB/Apo A1)in the case group is significantly higher than that of the control group(P<0.001),and the apolipoprotein in the case group A1(Apo A1),total cholesterol(TC),and low-density lipoprotein cholesterol(LDL-C)had lower levels than the control group(P<0.001).2.The distribution of SNPs at the three loci in the DPP4 gene in the case group and the control group is in accordance with Hardy Weinberg balance(rs3788989 χ~2 = 0.026,P = 0.871;rs4664443 χ~2 = 0.026,P = 0.871;rs13015258 χ~2 = 0.596,P = 0.742).There was no difference in DPP4 genotype and allele frequency distribution between the case group and the control group(P>0.05).In the control group,the serum ApoB(P=0.038)and ApoB/A1(P=0.040)levels of the DPP4 gene rs3788979 locus A allele mutation were lower than those of non-A allele carriers,and the difference was even greater in men.obvious.DPP4 gene rs13015258 locus genotype TT has higher ApoB(P=0.006)and ApoB/A1(P=0.023)levels than genotype GT,and genotype GG has higher ApoB/A1(P=0.039)than genotype GT Level.3.SNP genotypes at three loci of DPP4 gene were not found to be associated with the risk of T2DM combined with CAD(P>0.05).Conclusion: DPP4 gene rs3788979 locus A allele mutation reduced ApoB and ApoB/A1 levels,when confounding factors such as age,gender,smoking and BMI wereincluded by logistic regression used to analyze additive,dominant,recessive,and overdominant genetic models,but the risk of T2DM combined with CAD has not been reduced.