Study On The Regulatory Role Of Circular RNA Hsa＿circ＿0000326 In Malignant Biological Function And Clinical Significance Of Glioma

Object: To explore the effect of circular RNA(circ RNA)hsa＿circ＿0000326 on the biological function in glioma and its clinical significance.Methods: Firstly,in order to find a circ RNA,we analyzed the differential circ RNAs expression of glioma through a circ RNA chip(GSE146463)in the GEO database.Real-Time Quantitative Reverse Transcription Polymerase Chain Reaction(q RT-PCR)was used to detect the expression levels of hsa＿circ＿0000326 in glioma samples of 38 patients and normal brain tissues of 16 patients with traumatic brain injury.Meanwhile,we collected the clinical data onto patients,analyzing by Fisher’s exact test,Kaplan-Meier plot and COX regression model,to determine the relationship between hsa＿circ＿0000326 levels and prognosis of glioma patients,and explore its clinical significance.Secondly,hsa＿circ＿0000326 knockdown model was constructed using lentivirus and transfect in U251 and U87 cells.To investigate the impact of hsa＿circ＿0000326 on the biological function of glioma,we utilized many techniques,such as Cell Counting Kit-8(CCK-8),cell clone formation,cell scratch healing,Transwell chamber invasion and migration,flow cytometry,acridine orange(AO)staining and Western blot(WB).Finally,we used online databases and Cytoscape software to predict and construct a circ RNA-mi RNA-m RNA regulatory network.Results: qRT-PCR results showed that the expression level of hsa＿circ＿0000326was conspicuously increased in glioma tissues compared with normal brain tissues,and it was related to tumor grade.Kaplan-Meier curve showed that the overall survival of patients in hsa＿circ＿0000326 high expression group is relatively short.The COX regression suggested that the overexpression of hsa＿circ＿0000326(HR=5.462,95% CI(1.077-27.691),P=0.040)was a risk factor affecting survival in glioma patients.Cell experiments indicated that hsa＿circ＿0000326 knockdown could inhibit the proliferation,invasion,migration and autophagy of U251 and U87 cells,as well as promote cell apoptosis.Conclusions: Hsa＿circ＿0000326 was up-regulated in glioma tissues.It could promote the proliferation,invasion,migration and autophagy of glioma cells,as well as inhibit cell apoptosis Up-regulation of hsa＿circ＿0000326 is positively relevant to poor prognosis of glioma patients.And it might serve as a biomarker and target for clinical treatment of glioma.Hsa＿circ＿0000326 may function as competing endogenous RNAs to regulate the progression of glioma.