The Role Of Tumor Infiltrating Lymphocyte And Macrophages In The Progression And Prognosis Of Lung Adenocarcinoma

Background In recent years,immunotherapy has made remarkable breakthroughs in the field of lung cancer,but the overall objective remission rate of immunotherapy is not high.The relationship between tumor-infiltrating lymphocytes and macrophages in lung cancer and tumor prognosis is unclear.Objective To determine the distribution characteristics of CD4~+,CD8~+T cells and macrophages and their relationship with tumor progression and prognosis in lung adenocarcinoma.Methods Collect and follow up the patients diagnosed with lung adenocarcinoma after radical surgery in our hospital from January 2015 to December 2016.According to the size and prognosis of the patients,the paraffin slices of the tumor tissues were selected and stained with HE and CD4,CD8,CD68,CD 163 using immunohistochemistry.Results(1)Tumor-infiltrating CD4~+,CD8~+,CD68~+immune cells showed geospatial immune variability within the same patients and in different patients.There were more tumor infiltrating immune cells in the stroma than in the tumor.(2)Tumor progression was not related to the density of CD4,CD8,or CD68 positive immune cells infiltrated.(3)During the tumor progression,the immune cold area appeared and the increase in the number of immune cold area was independently related to poor prognosis.Conclusion In the evolution of lung adenocarcinoma,as the number of immune cold area increase,the prognosis becomes gradually worse.The spatial differential distribution of immune cells infiltrated in the tumor microenvironment may indicate the subclone evolution of the tumor,therefore is related to the underlying prognosis of different patients.