Expression Of KLKB1 In Plasma Of Patients With Colorectal Cancer And Its Clinical Significance

Objective:In 2020,China has 560,000 new cases of colorectal cancer and 290,000 deaths.The incidence of maligent turoms rate ranks second,and the mortality rate ranks fifth.Its high morbidity and high mortality rate pose a serious threat to human health.At present,there is no ideal blood biomarker for the diagnosis of colorectal cancer.In our own lab previous study,bioinformatics analysis found that plasma kallikrein is a key core gene in the colorectal cancer pathway,suggesting that it may be involved in the occurrence and development of colorectal cancer.The study was aim to detect plasma kallikrein concentrations in patients with colorectal cancer and to investigate the correlation between clinicopathologic features of colorectal cancer and plasma levels of KLKB1,providing a new target for the diagnosis and treatment of colorectal cancer.Methods:Colorectal cancer patients who underwent sugery of colorectal cancer in the Afiliated Hospital of Chengde Medical College from Octotber 2019 to Octotber 2020 were collected as the experimental group,and healthy volunteers during the same period served as the control group.The plasma KLKB1 concentrations was detected by enzyme-linked immunosorbent assay and serum CEA was dectected by immunoflunosorescence method.Analyze the relationship between the expression levels of KLKB1 and CEA in the blood and clinicopathologic characteristics and the correlation between the expressions of the two.Result:1.Comparison of the expression levels of KLKB1 in the plasma of colorectal cancer patients and healthy volunteers.The content of KLKB1 in the plasma of colorectal cancer patients(4.03±1.14U/m L)was higher than that of healthy controls(2.47±0.81U/m L),the difference was statistically significant(P<0.05).2.Comparison of the expression levels of CEA in the serum of colorectal cancer patients and healthy volunteers.The CEA content in the serum of patients with colorectal cancer(7.58±3.31ng/m L)was higher than that of healthy controls(4.04±1.80ng/m L),the difference was statistically significant(P<0.05).3.The correlation between the expression of plasma KLKB1 and clinicopathological characteristics in patients with colorectal cancer.The concentration of KLKB1 in the plasma of patients with colorectal cancer was detected by ELISA.The results showed that the content of KLKB1 in the plasma of patients with lymph node metastasis(4.39±1.00U/m L)was higher than that in patients without lymph node metastasis(3.65±1.17U/m L),the difference was statistically significant(P=0.008);the content of KLKB1 in the plasma of patients with distant metastasis(5.16±1.32U/m L)was significantly higher than that of patients without distant metastasis(3.85±1.01U/m L),the difference is statistically significant(P=0.001);In the clinical stageing of colorectal cancer,the level of KLKB1 in the plasma of patients with stage III-IV(4.44±1.04U/m L)is higher than the level of KLKB1 in the plasma of patients with stage I-II(3.61±1.11U/m L),the difference was statistically significant(P=0.004).There was no statistical difference between the concentration of KLKB1 in the plasma of patients with colorectal cancer and the expression of gender,age,tumor diameter,tumor growth location,and tumor invasion depth(P>0.05).4.The correlation between the expression of serum CEA and clinicopathological characteristics in patients with colorectal cancer.The serum CEA content of patients with colorectal cancer was detected by immunofluorescence method.The results showed that the serum CEA content of patients with distant metastasis(10.15±2.38U/m L)was significantly higher than that of patients without distant metastasis(7.17±3.27ng/m L),the difference was statistically significant(P=0.011).There was no significant difference between the CEA content in the patients’ serum and the expression of gender,age,tumor diameter,tumor growth location,tumor invasion depth,lymph node metastasis,and clinical stage(P>0.05).5.The correlation between the expression levels of KLKB1 and CEA in the blood of patients with colorectal cancer.The results of correlation analysis showed that there was no correlation between the concentration of KLKB1 and the content of CEA in the blood of patients with colorectal cancer(r=0.227,P=0.069).Conclusion:The expression of KLKB1 in the plasma of patients with colorectal cancer is elevated,and it is correlated with the clinical staging,lymph node and distant metastasis of colorectal cancer,but has no correlation with the traditional index CEA expression.It may become an independent test for colorectal cancer screening index.