Synthesis And Properties Of Curcumin/Gelatin/ Hyaluronicacid Composite Hydrogel

Objective:At present,it can be seen clinically that many patients after skin trauma and surgery have poor wound healing and long healing time.In order to accelerate the healing of patients’ wounds,researchers have conducted a large number of studies on surgical wound dressings in recent years.Compared with traditional wound dressings,cotton balls,cotton pads and gauze,etc.for their airtight,water removal and easy infectiousness,water gel dressings have received extensive attention and research in recent years.Because hydrogels have strong hydrophilicity and continuously absorb wound exudate,hydrogels have a broad prospect.Turmeric turmeric extract of Chinese traditional medicine known as a good anti-inflammatory,oxidation resistance,accelerate wound healing function,this article mainly graduate preparation load of curcumin/gelatin/hyaluronic acid(Gel-HA-Cur)hydrogel and its physical properties,slow-release drug properties,antimicrobial properties,biocompatibility,for water Gel material can be load for traditional Chinese medicine provides the theory basis for clinical.Methods: Gelatin,hyaluronic acid and 1-(3-dimethylaminopropyl)-3-ethylcarbodiimine hydrogels with different proportions were prepared by chemical crosslinking method,and the hydrogels loaded with/curcumin/gelatin hyaluronic acid were mixed into curcumin solution by physical method during the crosslinking process.The morphology and chemical results of hydrogel materials were characterized by SEM and FTIR.The swelling rate,degradation rate and porosity of hydrogels were measured by mass method.UV was used to determine the release amount of curcumin by measuring the absorption value of single point.S.aureus and E.coli were used to detect the antibacterial properties of hydrogels.CCK-8 and immunofluorescence were used to observe the proliferation and adhesion of L929 mouse fibroblasts to determine the biocompatibility.Results: 1.SEM results showed that the synthesized Gel-Ha-Cu R hydrogel had a good porous structure and transparency.2.FTIR can show that the amine group of Gel and the carboxyl group of HA have been successfully aminated to form the covalent bonds of Gel-HA,Gel-Gel and HA-HA.3.Drug release test showed that with the increase of gelatin content,the amount of curcumin released gradually increased,and the release amount of 20% Gel-Ha-Cur was the largest,the cumulative release amount was 14 ug,and the release time was 144 h.4.The antibacterial experiment showed that the Gel ha-cur hydrogel had certain antibacterial properties,and the hydrogel had no bacterial proliferation within 12 hours,but it was still insufficient to inhibit the growth of Escherichia coli and Glucobacteria aureus.5.Experimental study on cytocompatibility:with the increase of curcumin content,the relative proliferation rate of L929 mouse fibroblasts increased gradually,and the relative proliferation rate reached the highest at0.50mg/ m L and reached 124.2% on the first day.Therefore,it is possible that increasing the content of curcumin can promote the proliferation and adhesion of cells,but when the concentration increases to a certain level,there will be inhibition,and the relative value-added rate reaches 98.5% on the second day and 95.5% on the third day.Conclusions: The hydrogel material loaded with curcumin/gelatin/hyaluronic acid(Gel-HA-Cur)has good hydrophilicity and water absorption.Specific good porosity;Good loading capacity of turmeric and achieve the purpose of slow release;The material is non-cytotoxic and has good biocompatibility.It is hoped that it can provide help for patients who are difficult to heal after trauma in the future.Moreover,we hope that this study can provide theoretical basis and ideas for the combination of traditional Chinese medicine and modern medicine.