The Effect Of Peripheral Blood Leukocyte Telomere Length And Telomerase On The Prognosis Of Patients With Non-small Cell Lung Cancer

Objective:To explore the relationship between the prognosis of patients with non-small cell lung cancer and the telomere length and telomerase activity of peripheral white blood cells.Methods:This study adopted a prospective research design and selected 55 patients who were diagnosed with NSCLC from April 2016 to June 2017 in the Department of Cardiothoracic Surgery of Baoding First Central Hospital and whose clinical data were complete and met the enrollment criteria.According to the specific values of telomerase activity and telomere DNA length,they were divided into 4 groups.There are 7 cases in the long-chain telomerase DNA and high-activity telomerase group,14 cases in the long-chain telomere DNA and low-activity telomerase group,14 cases in the short-chain telomere DNA and low-activity telomerase group,and 20 cases in the short-chain telomere DNA and high-activity telomerase group.The general information of the patients was collected.The real-time fluorescence quantitative PCR method was used to detect the telomere length of peripheral blood leukocytes in patients with non-small cell lung cancer,then follow up the patient’s survival status,and finally analyze the relationship between the patient’s telomere length,telomerase activity and the patient’s prognosis.Results:The pathological type of tumor,gender and smoking have no obvious relationship with the survival time of patients(P>0.05).The overall TNM staging of the 4 groups was statistically significant(P=0.025).There was no correlation between telomerase activity and telomere length in each group members themselves(r=-0.112,P=0.228).The survival time of NSCLC patients in different groups: The survival time of patients in the long-chain telomere DNA and low-activity telomerase group was(30.93±1.06)months;the survival time of patients in the long-chain telomere DNA and high-activity telomerase group was(29.48±2.64)months;the survival time of patients with short-chain telomere DNA and high-activity telomerase group was(14.70±1.65)months;the survival time of patients with short-chain telomere DNA and low-activity telomerase group was(19.98±1.68)months.Overall comparison of prognostic survival time(OS)of patients in different groups has statistical significance(P<0.001).There was no statistically significant comparison of telomerase and telomere length in each group(P<0.05).Using Kaplan-Meier curve to compare the prognostic survival time of patients between groups,it is found that the overall comparison is statistically significant,and the comparison between the different groups :the comparison of the long-chain telomere DNA high-activity group and the long-chain telomere DNA low-activity group.There was no significant difference between the short-strand telomere DNA high-activity group and the short-strand low-activity group,and the comparison between the telomere DNA long-strand low-activity group and the telomere short-strand low-activity group(P>0.05).The telomere DNA was long.The comparison between the high-strand activity group and the short-strand high-activity telomere DNA group,and the low-activity long-strand telomere DNA group and the high-activity telomere DNA short-strand group showed statistical significance(P<0.05).According to Cox risk proportional regression analysis,age,TNM stage,telomere length,and telomerase activity are independent risk factors that affect the survival of NSCLC patients.Conclusion:The age,clinical TNM stage,telomere length of peripheral blood leukocytes,and telomerase activity in patients with non-small cell lung cancer can all be used as independent risk factors for patient prognosis.Among them,the change of telomere length has a significant effect on the prognosis of patients.