A Study On The Application Of Nonlinear Chemical Fingerprint In Preliminary Quality Control Of Rhodobryum Giganteum (Hook.) Par. And Gelatinous Chinese Medicine

Nonlinear chemical oscillation fingerprint of traditional Chinese medicine,as an analytical method to evaluate the overall redox activity of traditional Chinese medicine,plays a more and more important role in the quality control of traditional Chinese medicine.This paper is mainly divided into four parts.chapter 1 investigated the application of nonlinear chemical oscillation fingerprint in the identification of the base source of Artemisia angustifolia.In chapter 2,a method of nonlinear chemical oscillation fingerprint was established to evaluate the quality of animal gelatin,including the identification of E’jiao from different manufacturers,the identification of different kinds of gelatin sources,the quality consistency evaluation of different batches of E’jiao from the same factory,and so on.In chapter 3,the disturbance mechanism of E’jiao participating in chemical oscillatory reaction was explored.In the fourth part,the introduction of chemical oscillations and the application of nonlinear chemical oscillation fingerprint analysis are reviewed.1.Qualitative analysis of Rhodobryum giganteum（Hook.）Par.by using of the nonlinear oscillation chemical fingerprintIn order to improve the quality control standard of Rhodobryum giganteum（Hook.）Par.,a new method for qualitative and quantitative analysis of Rhodobryum giganteum（Hook.）Par.was established by using the nonlinear oscillating chemical in this paper.The potential（E）/time（t）curve was then recorded in the oscillation reaction system of BrO₃^--Ce（SO₄）₂-H₂SO₄-malonic acid/tartaric acid.The reproducibility of this method was investigated and the RSD values of the four characteristic parameters of Rhodobryum giganteum（Hook.）Par.were less than 4.12%.It should be noted that this fingerprint has good repeatability and precision.After optimizing the experimental parameters such as particle size,rotation speed and temperature,a new method based on nonlinear oscillating chemical can be used for qualitative and quantitative analysis of Rhodobryum giganteum（Hook.）Par.It showed that there were two good linear relationships between the induction time the period of oscillation and the dosage of herbs with the relative coefficients 0.978 and 0.975,respectively.Besides,the highest potential showed a good nonlinear relationship with the dosage of herbs.The method can be used to describe the Rhodobryum giganteum（Hook.）Par.and Rhodobryum roseum（Weis.）Cimpr.by the evidence differences in the oscillation time,the highest potential,the maximum amplitude and the induction time.Importantly,the significant difference was attributed to the induction time,which can be considered as a significant parameter in the discrimination of Rhodobryum giganteum（Hook.）Par.and Rhodobryum roseum（Weis.）Cimpr.2.A study on identification and analysis of gelatinous Chinese medicine based on nonlinear chemical oscillation fingerprintThe E-t curve of oscillating system over time is recorded,when gelatinous Chinese medicine are added to the acetone-BrO₃^--Mn²⁺-H₂SO₄ oscillating system.Nonlinear chemical oscillation fingerprints of samples were analyzed and contrasted each other by the two data analysis methods of Principal Component Analysis（PCA）and system similarity.It was turn out that similar results from such methods were reached,both of them can be used to identify ASINI CORII COLLA（E’jiao）produced by different manufacturers（characteristics of regional distribution）,and the gelatin of different provenance including TESTUDINIS CARAPACIS ET PLASTRI COLLA and CERVI CORNUS COLLA.Moreover,relative distribution of initial potential and equilibrium potential in E’jiao from 2007 to 2018,to some extent,provides reference for determining if the sample is within the five-year shelf life from that of other years.Dramatic quality difference was clearly displayed in both of data analysis,which remained us the necessity to control the gelatin making process.Therefore,this method is simple to operate,low cost and short time,and can provide a new idea for the existing quality control methods of GCM.3.Study on the mechanism of nonlinear chemical oscillation fingerprint for the identification of E’jiaoIn order to further explore different manufacturer gelatin similarity is low,the quality difference is bigger,the induction time,amplitude,cycle,oscillation on the cause of the difference characteristic parameters such as life,first of all,under the longer acid hydrolysis time,the analysis and comparison for the company in Shandong and Beijing Tongrentang the two different manufacturers the change law of acid hydrolysis of gelatin protein.Secondly,the effects of different amino acids on gelatin were compared from the point of view of amino acids,The effects of different amino acids on the oscillating system of E’jiao-Mn²⁺-BrO₃^--H⁺-CH₃COCH₃ were compared under the same order of magnitude,the disturbance of different amino acids to the oscillation system is different.Although the contents of tyrosine and cystine are the lowest,they have the greatest influence on the oscillation curve.Tyrosine significantly reduces the amplitude,and has a new oscillation mode,the period is longer,and the number of oscillations decreases obviously.Cystine significantly increases the amplitude and then suppresses the oscillation.Glycine and hydroxyproline with the highest content significantly reduce the amplitude,while alanine,proline and lysine make the amplitude move down as a whole.And there is a linear relationship between the amount of glycine and the amplitude decrease,ΔE=0.31m+0.44（R²=0.97）.Finally,the possible mechanism of amino acid disturbance to the oscillating system is explored by cyclic voltammetry.