Study On Lysosome-Targeted Tumor Synergistic Phototherapy Based On Ceria Nanozymes Drug Delivery System

Photodynamic therapy(PDT)is an emerging non-invasive cancer treatment method.In tumor coordinated phototherapy,how to accurately use tumor hypoxia and micro-acid environment to improve breast cancer treatment effect is worthy to consider.Based on the issues,this work proposed to construct a hollow ceria nanozyme drug delivery system(ATPHCNPs@Ce6)modified by adenosine 5’-triphosphate disodium salt hydrate(ATP)to deliver the photosensitizer Ce6 through the one-pot hydrothermal method and nano self-assembly synthesis technology for lysosome-targeted tumor synergistic photodynamic therapy.First,the hollowness of the hollow ceria nanozyme(HCNPs)was dependent on the concentration of nitric acid in the precursor.Secondly,the hollow ceria nanozyme carrier(ATP-HCNPs)could not only be used as a powerful drug carrier but also exhibit excellent biocompatibility and stability.Besides,as a modulator of enzymatic activity,ATP-HCNPs enable themselves to serve as an in situ oxygen-generating agent via utilizing the tumor microenvironment,which gave them the potential for synergistic optical therapy.In fact,ATP-HCNPs@Ce6 could achieve a selective oxygen supply strategy by responding to the highly expressed hydrogen peroxide at the tumor and the slightly acidic environment of tumor lysosomes,thereby enhancing lysosome-targeted photodynamic therapy.Finally,the obvious anticancer effects and high biological safety of ATP-HCNPs@Ce6 were demonstrated in vitro and in vivo experiments.In general,a simple and rapid self-assembly strategy for the preparation and modification of multifunctional ATP-HCNPs@Ce6 was proposed.And the corresponding tumor collaborative phototherapy mechanism of ATP-HCNPs@Ce6 was also fully proved,which laying a certain foundation for further promoting its application in the field of breast cancer precision treatment.