Effect Of Electroacupuncture On Autophagy Factors And Liver Lipid Metabolism In Rapidly Aging Mice

ObjectiveTo study the effect of electroacupuncture on the expression of autophagy-related factors LC3-Ⅱ,Beclin1,Atg7,and p62 in the liver of rapidly aging（Senescence accelerated mouse/prone8,SAMP8）mice,and to explore the mechanism of electroacupuncture to improve liver lipid metabolism in mice.MethodsThirty-week-old male SAMP8 mice were randomly divided into a model group,a drug group,and an electroacupuncture group,with 7 mice in each group.7 anti-rapid aging SAMR1 mice of the same age were used as the control group.The animals in the control group and the model group were bred routinely for 2 weeks without any intervention;the drug group was treated with intraperitoneal injection of rapamycin(10mg·kg^-1·d^-1)（once a day,6 consecutive days a week）;the electroacupuncture group was given"Shenshu"and"Taichong"Electroacupuncture at point（15minutes a day,6consecutive days a week）.Detection of mice serum lipid metabolism and liver lipid deposition,the distribution of liver autophagy body,liver LC3-Ⅱ,Beclin1,Atg7,p62 protein expression and the m RNA expression of changes.ResultsCompared with the control group,the total cholesterol（TC）,triglycerides（TG）,and low density lipoprotein（LDL）of the model group increased significantly（P<0.01）,In the model group,lipid droplet deposition was obvious,autophagosomes decreased,autophagy-related factors LC3-Ⅱ,Beclin1,Atg7 protein and m RNA expression levels were significantly decreased（P<0.01）,p62 protein and m RNA were significantly increased（P<0.01）.Compared with the model group,the serum TG,TC,and LDL contents of the mice in the electroacupuncture group and the drug group were significantly reduced（P<0.01）,lipid droplet deposition was reduced,autophagosomes increased,LC3-Ⅱ,Beclin1,Atg7 protein and m RNA expression levels increased significantly（P<0.01）,and p62 protein and m RNA expression levels decreased significantly（P<0.01）.The expression levels of Beclin1,Atg7 protein and m RNA in the liver of the electroacupuncture group were not significantly different from the drug group（P>0.05）.ConclusionElectroacupuncture can reduce liver lipid metabolism disorders,which may be related to the regulation of the expression of liver autophagy related factors LC3-Ⅱ,Beclin1,Atg7,and p62,thereby promoting liver autophagy in SAMP8 mice.