Study On The Dose-Effect-Time-Effect Relationship Of Wild Jujube And Jujube Seed In Antagonizing Anxiety

Objective:Through experimental research,the dose-effect and time-effect relationship of wild jujube’s antagonistic effect on state anxiety in rats are clarified,the dose-effect and time-effect relationship of wild jujube seed in antagonizing state anxiety in rats,and the relationship between wild jujube and wild jujube is discussed Compatibility of kernels antagonizes the difference of state anxiety in rats.Methods:Part Ⅰ: Study on the dose-effect-time-effect relationship of wild jujube antagonizing state anxiety in rats1.Study on the dose-effect relationship of wild jujube antagonizing state anxiety in ratsSeventy male SD rats were selected and adapted to the environment for 7 days.They were randomly divided into a model control group,a blank control group,and different doses of wild jujube administration groups: wild jujube 1,wild jujube 2,wild jujube 3,wild jujube 4,wild jujube 5 Groups of 10 animals per group.The model control group and the different doses of jujube administration group were placed in a conditioned fear stimulus box to give sound stimulation(75 d B;1000 Hz;5 s)and electrical stimulation(0.5 m A;1s)during the last 1s of the sound signal.The interval between the two stimulations was 60 s,and a total of 15 cycles were performed.The blank control group was given only sound stimulation but not electric stimulation.According to the above method,the model was made for 4 consecutive days.On the day after the model was replicated,the different doses of jujube administration group were given 1.25 g/kg,2.5 g/kg,5 g/kg,10 g/kg,20 g/kg(equivalent to crude drug amount)via intragastric administration.The lyophilized powder of wild jujube was administered continuously for 10 days.The model control group and the blank control group were given the same volume of distilled water.The EPM experiment was performed 1 hour after the last administration,and EPM behavior related indicators were recorded and analyzed by video.2.Study on the time-effect relationship of wild jujube antagonizing state anxiety in ratsSeventy male SD rats were selected and adapted to the environment for 7 days.They were randomly divided into a model control group,a blank control group and a wild jujube administration group for different administration days: wild jujube 5 days group,wild jujube 7 days group,wild jujube 9 days group,wild jujube 11-day group,each group has 10 animals.The model control group and the jujube administration group with different administration days were placed in a conditioned fear stimulus box to give sound stimulation(75 d B;1000 Hz;5 s)and electrical stimulation(0.5m A)during the last 1 s of the sound signal.;1 s),the interval between every two stimulations is 60 s,a total of 15 times.The blank control group was given only sound stimulation but not electric stimulation.According to the above-mentioned method,the model was made for 4 consecutive days.On the day after the model was replicated,the wild jujube administration groups with different administration days were given 5g/kg(equivalent to crude drug amount)of wild jujube freeze-dried powder via gavage for 5 days,7 days,9 days,On the 11 th day,the model control group and the blank control group were given the same volume of distilled water.The EPM experiment was performed 1 hour after the last administration,and EPM behavior related indicators were recorded and analyzed by video.Part Ⅱ : The dose-effect-time-effect relationship of jujube seed in antagonizing state anxiety in rats1.The dose-effect relationship of jujube seed in antagonizing state anxiety in ratsSeventy male SD rats were selected and adapted to the environment for 7 days.They were randomly divided into a model control group,a blank control group,and different doses of jujube seed administration groups: jujube seed 1 group,jujube seed2 group,and jujube seed 3 Group,4 groups of raw jujube kernels,5 groups of raw jujube kernels,10 in each group.The model control group and the different doses of jujube seed administration group were placed in a conditioned fear stimulation box to give sound stimulation(75 d B;1000 Hz;5 s)and electrical stimulation(0.5 m A;1s)during the last 1s of the sound signal.The interval between every two stimulations was 60 s,and there were 15 cycles in total.The blank control group was given sound stimulation but not electric stimulation.According to the above method,the model was made for 4 consecutive days.On the day after the model was replicated,different doses of jujube seed administration group were given 1.25 g/kg,2.5 g/kg,5 g/kg,10g/kg,20 g/kg(equivalent to crude drug amount)via intragastric administration.)Freeze-dried powder of raw jujube kernels,administered continuously for 10 days,and the model control group and the blank control group were given the same volume of distilled water.The EPM experiment was performed 1 hour after the last administration,and EPM behavior related indicators were recorded and analyzed by video.2.Study on the time-effect relationship of jujube seed antagonizing state anxiety in ratsSeventy male SD rats were selected and adapted to the environment for 7 days.They were randomly divided into a model control group,a blank control group,and a jujube seed administration group with different administration days: jujube seed 5days group,jujube seed 7 days group,The 9-day-old jujube group and the 11-day-old jujube group had 10 animals in each group.The model control group and the jujube seed administration group of different administration days were placed in a conditioned fear stimulation box to give sound stimulation(75 d B;1000 Hz;5 s)and electrical stimulation(0.5 m A;1 s),each stimulation interval is 60 s,a total of 15 times.The blank control group was given only sound stimulation but not electric stimulation.According to the above method,the model was continuously made for 4days.On the day after the model was replicated,the jujube seed administration groups with different administration days were given 5 g/kg(equivalent to the crude drug amount)of freeze-dried jujube seed powder by gavage for 5 days and 7 days respectively.On days 9 and 11,the model control group and the blank control group were given the same volume of distilled water.The EPM experiment was performed 1hour after the last administration,and EPM behavior related indicators were recorded and analyzed by video.Part Ⅲ: Study on the difference between wild jujube and raw jujube in antagonizing state anxiety in rats1.The difference of the dose-effect relationship between wild jujube and jujube seed in antagonizing state anxiety in ratsPut the dose-effect results of the first part of wild jujube and the second part of the dose-effect result of raw jujube in the same coordinate system,and compare the dose-effect difference of antagonistic state anxiety between wild jujube and raw jujube.2.Study on the time-effect relationship difference between wild jujube and raw jujube in antagonizing state anxiety in ratsPut the time-effect result of the first part of wild jujube and the second part of the time-effect result of raw jujube in the same coordinate system,and compare the time-effect difference of antagonistic state anxiety between jujube and raw jujube.Results:Part Ⅰ: Study on the dose-effect-time-effect relationship of wild jujube antagonizing state anxiety in rats1.Study on the dose-effect relationship of wild jujube antagonizing state anxiety in ratsThe experimental results show that compared with the blank control group,the number of open arms(n),percentage of open arms(%),open time(s),and percentage of open arm time(%)in the model control group in EPM are reduced,There is a significant difference(P<0.05,P<0.01),the time to close the arm(s)increases,and there is a very significant difference(P<0.01),the number of arms closed(n),the total number of arms entry(n),central There is no difference in grid dwell time(s).Compared with the model control group,the number of open arms(n),percentage of open arms(%),open time(s),percentage of open arm time(%),stay time in central grid(s)of rats in the jujube administration group)Increase with the increase of the dose,and the time to close the arm(s),the number of times to close the arm(n),and the total number of arm entrances(n)decrease with the increase of the dose.Among them,the open arm time(s)and open arm time percentage(%)of the three groups of wild jujube increased the most,and there was a significant difference between the two groups(P<0.05);the closed arm time decreased the most,and there was a significant difference between the two groups(P <0.05).2.Study on the time-effect relationship of wild jujube antagonizing state anxiety in ratsThe experimental results show that compared with the blank control group,the number of open arms(n),the percentage of open arms(%),the open time(s),the percentage of open arm time(%),The number of closed arms(n),the total number of arm entrances(n),and the staying time in the central grid(s)are reduced,and there is a significant difference between the two groups(P<0.05 or P<0.01);the time for rats in the model control group to enter the closed arm(S)Increase,and there is a significant difference between the two groups(P<0.05 or P<0.01).Compared with the model control group,the number of open arms(n),percentage of open arms(%),open arm time(s),percentage of open arm time(%),number of closed arms(n),The total number of arm entrances(n)and the stay time of the central grid(s)increase with the increase of time;the closing time(s)decreases with the increase of time.On the 5th day,the number of openings(n),The arm opening time(s)and the percentage of arm opening time(%)were significantly increased,and there was a significant difference compared with the model group(P<0.05 or P<0.01).Part Ⅱ : The dose-effect-time-effect relationship of jujube seed in antagonizing state anxiety in rats1.The dose-effect relationship of jujube seed in antagonizing state anxiety in ratsThe experimental results show that compared with the blank control group,the number of open arms(n),the percentage of open arms(%),the open time(s),the percentage of open arm time(%),The number of closed arms(n),the total number of arm entrances(n),and the staying time in the central grid(s)are reduced,and there is a significant difference between the two groups(P<0.05 or P<0.01);the time for rats in the model control group to enter the closed arm(S)Increase,and there is a significant difference between the two groups(P<0.05 or P<0.01).Compared with the model control group,the number of open arms(n),percentage of open arms(%),open arm time(s),percentage of open arm time(%),total number of arm openings of rats in the Shengzao kernel administration group(N),the stay time of the central grid(s)increases with the increase of the dose,and the time to close the arms(s),the number of times(n),decreases with the increase of the dose.Among them,the arm opening time(s),arm opening time percentage(%),arm opening frequency(n),and arm opening frequency percentage(%)increased the most in the three groups of raw jujube kernels.There was a very significant difference between the two groups(P< 0.01);the time to close the arm was reduced the most,and there was a very significant difference between the two groups(P<0.01).2.Study on the time-effect relationship of jujube seed antagonizing anxiety in ratsThe experimental results show that compared with the blank control group,the number of open arms(n),the percentage of open arms(%),the open time(s),the percentage of open arm time(%),The number of closed arms(n),the total number of arm entrances(n),and the staying time in the central grid(s)are reduced,and there is a significant difference between the two groups(P<0.05 or P<0.01);the time for rats in the model control group to enter the closed arm(S)Increase,and there is a significant difference between the two groups(P<0.05 or P<0.01).Compared with the model control group,the number of open arms(n),percentage of open arms(%),open arm time(s),percentage of open arm time(%),number of closed arms(n),The total number of arm entrances(n)and central grid residence time(s)increase with the increase of time;the closed arm time(s)decreases with the increase of time.Among them,the number of open arms(n),the percentage of open arms(%),the open time(s),and the percentage of open time(%)are significantly increased,and there is a significant difference compared with the model group(P<0.05 or P<0.01),and the drug is effective;At 11 days of continued administration,the number of open arms(n),percentage of open arms(%),open time(s),and percentage of open arm time(%)increased significantly,and there was a significant difference compared with the model group(P<0.05 or P<0.01).Part Ⅲ: Study on the difference between wild jujube and raw jujube in antagonizing state anxiety in rats1.The difference of the dose-effect relationship between wild jujube and jujube seed in antagonizing state anxiety in ratsThe experimental results show that when the open arm time(s),the percentage of open time(%),the number of open arms(n),and the percentage of open arms(%)are used as indicators for evaluation,the value is between 1.25 g/kg-20 g/ Within the kg dose range,the effect of jujube seed on antagonizing state anxiety in rats is generally stronger than that of jujube.2.Study on the time-effect relationship difference between wild jujube and raw jujube in antagonizing state anxiety in ratsThe experimental results show that when the open arm time(s),the percentage of open arm time(%),the number of open arms(n),and the percentage of open arms(%)are used as indicators for evaluation,the wild jujube antagonizes The effect of state anxiety in rats is generally stronger than that of jujube seed;in the 7-11 days,the effect of jujube seed in antagonizing state anxiety in rats is generally stronger than that of jujube.Conclusion:1.Jujube has the effect of antagonizing state anxiety in rats.The best dosage is 5 g/kg,and the effect is best by intragastric gavage for 5 days.2.Jujube seed has the effect of antagonizing state anxiety in rats,the best dosage is 5g/kg,and the effect is best by continuous intragastric administration for 11 days.3.Jujube seed has a significant antagonistic effect on state anxiety in rats,which is better than wild jujube.