Determination Of 25-hydroxyvitamin D Level In Children With Epilepsy And Its Correlation With C-reactive Protein And Electrolyte

Objective: To explore the relationship between 25-OH-VD level and CRP,electrolyte level in children with epilepsy of different seizure duration,and to further understand the role of 25-OH-VD,CRP and electrolyte in the pathogenesis of children with epilepsy.Methods: From January 2019 to June 2020,83 children with epilepsy who met the clinical practical definition of epilepsy of ILAE in 2014 were selected and divided into three groups according to their seizure duration: 43 cases with seizure time < 5 minutes,30 cases with seizure time 5-30 minutes and 10 cases with seizure time ≥30 minutes.Meanwhile,30 healthy cases were randomly selected at the same time.Two 2ml venous blood samples were collected from children in epilepsy group immediately after the seizure stopped,and the same amount of venous blood samples were collected from healthy children in the control group.ELISA was used to detect 25-OH-VD,immunoturbidimetry was used to detect CRP,and ion-selective electrode method and arsenazo Ⅲ method were used to detect electrolyte level.SPSS21.0 was used for statistical analysis of the data.The 25-OH-VD,CRP and electrolyte levels of the children in each group were compared,and the correlation between 25-OH-VD and other laboratory indicators was analyzed.Results: 1.Compared with the control group,the levels of 25-OH-VD,serum sodium and serum calcium in the epilepsy group were decreased(P <0.05).2.Compared with the control group,the levels of serum potassium and CRP in the epilepsy group were increased(P < 0.05).3.With the prolongation of seizure time,the levels of 25-OH-VD,serum sodium and serum calcium in children with epilepsy group decreased gradually(P < 0.05).4.With the prolongation of seizure time,serum potassium and CRP levels in children with epilepsy group were gradually increased(P < 0.05).5.The level of25-OH-VD was positively correlated with serum sodium and calcium in epilepsy group(r = 0.272,P < 0.05;r = 0.263,P < 0.05).6.The level of25-OH-VD was negatively correlated with serum potassium and CRP in epilepsy group(r =-0.278,P < 0.05;r =-0.357,P < 0.05).Conclusions:1.The level of 25-OH-VD in epileptic group was lower than that in control group,which showed that epileptic seizure affected the level of25-OH-VD in epileptic group,suggesting that 25-OH-VD participated in the pathophysiological process of epileptic seizure.2.The levels of serum sodium and calcium in children with epilepsy were lower than those in control group,but the levels of serum potassium were higher than those in control group,which indicated that epilepsy affected the electrolyte level of children,suggesting that serum potassium,serum sodium and serum calcium participated in the pathophysiological process of epilepsy.3.The 25-OH-VD level decreases progressively with the prolongation of epileptic seizure time,which indicates that the longer the epileptic seizure duration,the greater the influence on the children’s 25-OH-VD level.It is suggested that the determination of 25-OH-VD level in children with epilepsy can be used as an index to reflect the severity of epilepsy.4.The levels of serum sodium and calcium decrease progressively with the prolongation of seizure time,while the level of serum potassium increases progressively with the prolongation of seizure time,which indicates that the longer the duration of seizure,the greater the influence on the levels of serum potassium,serum sodium and serum calcium in children,and the higher the probability of electrolyte disturbance.It is suggested that attention should be paid to monitoring the levels of serum potassium,serum sodium and serum calcium in children with seizure,which can reflect the severity of seizure.5.The level of CRP in children with epilepsy is higher than that in the control group,and increases progressively with the prolongation of seizure time,which shows that the longer the seizure time,the more obvious the acute injury in children with epilepsy,suggesting that CRP can be used as an index to reflect the evaluation of acute injury after seizure.6.The level of 25-OH-VD in children with epilepsy is positively correlated with serum sodium and calcium,and negatively correlated with serum potassium,indicating that 25-OH-VD may participate in the process of epileptic seizure by regulating the changes of electrolyte level in the body.7.The level of 25-OH-VD in children with epilepsy is negatively correlated with CRP,suggesting that 25-OH-VD may be a potential index reflecting neuroinflammatory reaction and severity of acute injury in children with epilepsy.