Expression And Clinical Significance Of MiR-127,miR-221and MiR-223 In Neonatal Pneumonia

Objective: To explore the changes in the expression levels of miRNAs(miR-127,miR-221,miR-223)in the plasma of neonates with pneumonia,so as to provide reliable biomarkers for the diagnosis of neonatal pneumonia,and that can also provide theoretical basis for the further study of the relationship between miR-127,miR-221,miR-223 and neonatal pneumonia.Methods: The clinical data of 20 neonates hospitalized in the Neonatal Intensive Care Unit of our hospital from March 2020 to October 2020 were collected.All into the group of newborns were admitted to hospital within 6 hours after birth and delivered by cesarean section,Apgar score ≥4points at the first minute of birth,without congenital or hereditary disease,or severe birth asphyxia,and their mothers had no antibiotic treatment and autoimmune diseases before delivery.According to the diagnostic criteria of pneumonia,10 neonates with neonatal pneumonia were selected,and 10 neonates without lung disease or infectious disease were selected as the control group.Plasma samples of the pneumonia group(before treatment)and the control group were collected respectively before antibiotics therapy.Plasma samples of the pneumonia group(before treatment)were collected again after 7 days of treatment,and were evacuated ventilator or without oxygen support when the plasma was collected again.Finally,RT-q PCR was used to detect miRNAs,and the expression levels of miR-127,miR-221 and miR-223 in plasma of the pneumonia group(before treatment)and the control group were compared.And then comparing the expression levels of miR-127,miR-221 and miR-223 in plasma before and after treatment of the pneumonia group.Results: There was no significant difference in gestational age and body weight between the two groups(P>0.05).The expression level of miR-127 was significantly different among groups(P<0.01).Before anti-infection,the expression level of miR-127 in pneumonia group(before treatment)was significantly higher than that in control group,and the difference between the two groups was statistically significant(P<0.05).However,compared with the pneumonia group(before treatment),the expression level of miR-127 in the pneumonia group(after treatment)was significantly decreased,and the difference between the two groups was statistically significant(P<0.01).And there was no statistically significant difference in miR-127 expression between the pneumonia group(after treatment)and the control group(P>0.05).In addition,The expression level of miR-221 was significantly different among groups(P<0.01).The expression level of miR-221 in the pneumonia group(before treatment)was significantly different from that in the control group(P<0.01).Compared with the pneumonia group(before treatment),miR-221 level in the pneumonia group(after treatment)decreased,the difference was statistically significant(P<0.01),but there was no statistically significant difference compared with the control group(P>0.05).In addition,the expression level of miR-223 was significantly different among groups(P<0.001).The expression level of miR-223 in the pneumonia group(before treatment)was significantly different from that in the control group(P<0.01).Compared with the pneumonia group(before treatment),the level of miR-223 in the pneumonia group(after treatment)was significantly decreased(P<0.001),and the difference was not statistically significant compared with the control group(P>0.05).Conclusion: MiR-127,miR-221 and miR-223 may play a key role in the pathogenesis of neonatal pneumonia,and they are potential biomarkers of neonatal pneumonia.