The Labeling Of Neural Stem Cells With Magnetic Nano Iron Oxide Drug And Their Proliferation And Differentiation Under Medium-frequency Low Intensity Pulsed Magnetic Field Stimulation

Neurological diseases are characterized by the gradual death and loss of cells in the central nervous system of the human body,resulting in irreversible damage to the nervous system.Drug and surgical intervention have limited therapeutic effects on neurological diseases,but cell therapy based on stem cell transplantation has shown good therapeutic potential in clinical research.In order to evaluate the safety and therapeutic effect of stem cell transplantation,it is necessary to image stem cells transplanted into the body and track their migration and distribution in the body.The magnetic nano-iron oxide drug has good biocompatibility,has been approved for clinical application,and has unique physical and chemical properties.It can perform real-time,non-invasive and in vivo tracking of transplanted cells under magnetic resonance imaging(MRI).Therefore,neural stem cells labeled with nano-iron oxide drugs have important research value and application significance in clinic.Pulsed magnetic field has been applied to clinical nervous system regulation and treatment.Pulsed magnetic field of suitable frequency and intensity can affect the proliferation and differentiation of neural stem cells.Incorporating the stimulating and regulating effect of pulsed magnetic field in cell transplantation therapy will make it possible to further enhance the therapeutic effect and generate new therapeutic techniques.Therefore,this paper aims to study the method and safety of magnetic nano-iron oxide drugs as MRI tracers for labeling neural stem cells,and to preliminary explore the effect of pulsed magnetic field stimulation on stem cell proliferation and differentiation.The main work of this paper is using a superparamagnetic nano-iron oxide drug(trade name: Ruicun),which has been approved by the Chinese FDA clinical trial,to label neural stem cells in detail.The detailed characteristics of the labeled stem cells have approved that Ruicun can be efficient and safe.Furthermore,the effect of proliferation and differentiation of magnetic nano-iron oxide drug labeled neural stem cells under the stimulation of 5k Hz/3.5m T pulse magnetic field was detected.The main work of this paper includes the following two aspects:1.The addition of polylysine during the co-incubation and cultivation of Ruicun and neural stem cells effectively promotes the uptake of magnetic nano-iron oxide by neural stem cells.Through the detection of the cell morphology,cell viability,cell proliferation and differentiation ability of Ruicun labeled neural stem cells,it was verified that polylysine-mediated Ruicun labeled stem cells is a safe,efficient and concentration dependent cell labeling method.2.The ability of proliferation and differentiation of neural stem cells marked by Ruicun under the pulsed magnetic field stimulation of medium frequency and low intensity(5k Hz/3.5m T)was studied,.The proliferation and differentiation of stem cells exposed to pulsed magnetic field were examined differently depend on exposure time.It was confirmed that the proliferation ability of neural stem cells marked by Ruicun was stimulated by pulsed magnetic field of 5-10 min,and the stimulation of pulsed magnetic field of 5min promoted the differentiation of neural stem cells into neurons.It is confirmed that pulsed magnetic field stimulation has the potential to regulate the proliferation and differentiation of neural stem cells...