Effects And Dynamic Mechanism Of Transcranial Magnetic Acoustical Electrical Stimulation On Neuronal Discharge Activity

Transcranial magnetic acoustical electrical stimulation(TMAES)is a new type of non-invasive nerve regulation method,with deep stimulation depth and good spatial resolution,which has important significance for the study of its biological effects.In view of the critical position of neuronal discharge activity and ion channel activity in neural regulation,this article aims to explore the effect of TMAES on neuron discharge activity and potassium ion channel current,and reveal the mechanism of neuronal regulation on neurons.The main research contents are as follows:1.Based on the ML neuron model,the bifurcation analysis of the ML neuron is performed when the induced current generated by TMAES is used as the bifurcation parameter.When magnetic acoustical electrical stimulations of different intensities act on neurons,we can explore the conversion of discharge activity patterns in neurons,and find that magnetic acoustical electrical stimulation can excite resting neurons,which can lead to the emergence of multiple neuronal discharge activities.2.The whole-cell patch-clamp technique was used to record the changes of the firing activity of pyramidal neurons in the hippocampal CA1 area of rats after 10 min and 20 min of ultrasound stimulation,and magnetic acoustical electrical stimulation.First of all,this article explores the effect of TMAES on the excitability of hippocampal neurons.Experimentally collected spontaneous action potentials of neurons,and found that TMAES can significantly increase the discharge frequency of action potentials;collected neuronal evoked action potentials,The results show that TMAES can prolong the duration of ascending and descending branches of action potential,reduce the amplitude of action potential,and increase the frequency of neuron-evoked action potential.Then,the transient outward potassium current and delayed rectifier potassium current of the neuron were further collected.The results showed that,compared with the control group,the TMAES significantly reduced the amplitude of the transient outward potassium current and the delayed rectifier potassium current.The value indicates that TMAES increases the excitability of neurons,which may be caused by the suppression of transient outward potassium current and delayed rectifier potassium current through TMAES.In summary,TMAES increases the excitability of neurons,because it suppresses the transient outward potassium current and delayed rectifier potassium current which are critical to the action potential depolarization and repolarization.Current stimulation produced by the MAES also play an important role in the regulation of neurons.These results have important reference value for the development of neuromodulation technology and the development of clinical treatment methods in the future,and help to further explore more advanced new methods of magnetic acoustical electrical nerve control.