The Expression And Correlation Analysis Of SIRT-1 And HIF-1α In Synovium Of Different Stages Of Temporomandibular Joint Disorders

Objective: To detect the expression of SIRT-1(Silent information regulator 1)and HIF-1α(Hypoxia-inducible factor-1α)in different stages of temporomandibular joint disorder(TMD),and analyze the possible internal connection between them.Methods: A collection of 23 patients(28joints)who received treatment in the Affiliated Stomatological Hospital of China Medical University and were diagnosed with temporomandibular joint disorders from 2019 to 2020 was recorded.We collected medical history,clinical specialist examinations and auxiliary examinations(including MRI,CBCT,panoramic film),and divided our patient into three groups according to Wikes’ staging classification,group A: stage Ⅲ(8 joints),group B: stage Ⅳ patients(11joints),and group C: stage Ⅴ patients(9 joints).The hyperplastic synovium was taken as a tissue sample during the operation,and the expression levels of SIRT-1 and HIF-1protein were detected by Western blot and immunohistochemical methods.Use statistical methods to count and analyze the respective expression levels of the two factors in the synovium,the correlation between the two,and the correlation with clinicopathological parameters.Results: 1.The pathological changes of the synovial membrane play a great role in the clinical symptoms of the patient.The more severe the inflammation and congestion of the synovial membrane,the higher the patient’s pain.2.In the TMJ synovium of the three groups of patients with different stages,SIRT-1showed positive expression,and the expression of SIRT-1 protein in stage Ⅳ was significantly higher than that in stage Ⅲ,while the expression of stage Ⅴ protein was lower than that of stage Ⅲ and Ⅳ.Stage IV>Phase III>Phase V.3.In the TMJ synovium of the three groups of patients with different stages,HIF-1α was positively expressed,and the expression of HIF-1α in stage Ⅳ was significantly higher than that in stage Ⅲ,while the expression of stage Ⅴ protein was lower than that of stage Ⅲ and Ⅳ.It shows stage Ⅳ>stage Ⅲ>stage Ⅴ.4.There is a positive correlation between the expression of SIRT-1 and HIF-1α in synovial tissues of patients with stage III,IV,and V.Conclusions:The protein expression of SIRT-1 and HIF-1αin the synovial tissue of patients with stage III,IV,and V TMD showed a trend of increasing in the early and intermediate stages and then decreasing as the disease progressed to the late stage,which may be related to the degree of pain and the degree of synovial disease.In addition,the expression of the two in phases III,IV,and V is positively correlated,and may play an important synergistic role in regulating the occurrence and development of TMD.