Significance Of Lymphocyte Subsets In Children With Mycoplasma Pneumoniae Pneumonia Treated By Fiberoptic Bronchoscopy

Objective: To explore the laboratory indexes of lymphocyte subsets in children with Mycoplasma pneumoniae pneumonia(MPP)treated twice or more by fiberoptic bronchoscopy.Methods: A total of 116 children with MPP who were treated with fiberoptic bronchoscopy in our hospital from January 2018 to January 2020 were retrospectively analyzed.According to the times of fiberoptic bronchoscopy treatment,they were divided into single group and multiple group.The basic data,clinical characteristics,percentage of CD3 +,CD8 +,CD4 + T cells,percentage of CD56 +,CD19 + lymphocytes and ratio of CD4 + / CD8 + in peripheral blood and BALF were compared between the two groups.At the same time,during the first fiberoptic bronchoscopy treatment in the two groups,the percentage of CD3 +,CD8 +,CD4 + T cells,the percentage of CD56 +,CD19 + lymphocytes and the ratio of CD4 + / CD8 + in peripheral blood and BALF were compared.Results:1.There was no significant difference in gender and age between the two groups(P > 0.05).2.The incidence of sputum embolism under the first bronchoscopy in the multiple group was significantly higher than that in the single group(P < 0.05),but there was no significant difference in mucosal necrosis and stenosis between the two groups(P > 0.05).The proportion of refractory Mycoplasma pneumoniae pneumonia in the multiple group was significantly higher than that in the single group(P < 0.05),while the proportion of severe Mycoplasma pneumoniae pneumonia had no significant difference between the two groups(P > 0.05).3.In the peripheral blood lymphocyte subsets,there was no significant difference in the percentage of CD3 +,CD4 + T cells,CD4 + / CD8 +ratio and CD19 + lymphocytes between the two groups(P > 0.05),but the percentage of CD8 + T cells in the multiple group was higher than that in the single group(P < 0.05).However,in BALF lymphocyte subsets,the percentage of CD4 + T cells and the ratio of CD4 + / CD8 + in the multiple dose group were significantly lower than those in the single dose group,the percentage of CD3 +and CD8 + T cells in the multiple dose group were significantly higher than those in the single dose group,and the percentage of CD19 + lymphocytes in the multiple dose group was lower than that in the single dose group(P < 0.05).4.The percentages of CD3 + and CD8 + T cells in BALF lymphocyte subsets in single dose group and multiple dose group were higher than those in peripheral blood,the ratio of CD4 + / CD8 + and the percentage of CD19 + lymph nodes in BALF lymphocyte subsets in single dose group and multiple dose group were lower than those in peripheral blood(P < 0.05).The difference was that there was no significant difference in the percentage of CD4 + T cells in peripheral blood and BALF lymphocyte subsets in single dose group(P > 0.05),but the percentage of CD4 + T cells in BALF lymphocyte subsets in multiple dose group was significantly lower than that in peripheral blood(P < 0.05).Conclusion:1.Compared with peripheral blood,the activity of CD4 + T cells in airway was significantly inhibited and the abnormal activation of CD8 + T cells was obvious in children with MPP treated by two or more times of fiberoptic bronchoscopy.2.The percentage of CD4 + T cells in BALF lymphocyte subsets was significantly lower than that in peripheral blood,and the percentages of CD3 +and CD8 + T cells in BALF lymphocyte subsets were significantly higher than those in peripheral blood,accompanied by the decrease of CD4 + / CD8 + ratio and CD19 + lymphocyte percentage,and the percentage of CD8 + T cells in peripheral blood lymphocyte subsets was significantly higher,Two or more times of fiberoptic bronchoscopy treatment can be suggested.