Expression And Significance Of PHH3 In Urothelial Carcinoma Of Bladder

Objective : Bladder cancer is the most common urinary system tumor,the morbidity and mortality of urinary system tumor is always the first.In this study,through the expression of Phosphorylated Histone H3(PHH3)in normal bladder mucosal tissues and urothelial carcinoma tissues,the relationship between Phosphorylated Histone H3 expression and clinicopathological characteristics and prognosis in urothelial carcinoma was explored,and the influence of PHH3 on the prognosis of urothelial carcinoma was analyzed.Improve the quality of life of patients.Methods : Immunohistochemical method respectively were used to detect 87 cases of bladder urothelial carcinoma tissue specimens of paraffin wax and 40 cases of normal urinary PHH3 protein expression in the epithelial tissue,in combination with clinical data analysis of the expression and clinical stage,pathologic stage of the tumor,presence of lymph node metastasis and recurrence of postoperative patients with bladder tumor electricity statistical relationship between.Then,quantitative analysis of PHH3 protein in 40 fresh bladder urothelial carcinoma specimens and 40 normal bladder mucosa tissues was performed by Western blotting to investigate the relationship between PHH3 protein expression level and patients’ age,gender,clinical stage,pathological grade,lymph node metastasis and other clinical data.The significance of PHH3 protein expression level on tumor proliferative activity was further analyzed and prognosis was analyzed.Results:1.Immunohistochemical results showed that the PHH3 protein was located in the nucleus and was brownish yellow or tan.PHH3 expression was positive in 63 of 87 cases of urothelial carcinoma(72%)and in 3 of 31 cases of normal urothelial carcinoma(7%).The positive rate of PHH3 protein in urothelial carcinoma tissues was significantly higher than that in normal urothelial tissues,and the difference was statistically significant(P < 0.05).2.In urothelial carcinoma patients,the expression of PHH3 protein was significantly correlated with clinical stage,pathological grade,and lymph node metastasis(P < 0.05);There was no significant correlation with age and gender of patients(P > 0.05).3.Among patients with non-muscular-infiltrating bladder cancer,the positive expression rate of PHH3 in 29 patients in low-risk and medium-risk groups was 41%,and that in 10 patients in high-risk group was 100%.The positive expression rate in high-risk group was significantly higher than that in low and medium-risk groups,with statistical significance(P < 0.05).4.The 5-year recurrence free rate of patients with phh3 positive expression was35.7%,and that of patients with phh3 negative expression was 55.6%.Kaplan Meier analysis showed that there was no significant difference in the recurrence rate between the two groups(P > 0.05)5.Western blotting results showed that the expression level of PHH3 protein in bladder cancer tissues was higher than that in normal bladder mucosa tissues,and the difference was statistically significant(P < 0.05),and the expression level was significantly correlated with clinical stage,pathological grade and lymph node metastasis(P < 0.05).6.In the 40 patients with urothelial carcinoma,the expression of PHH3 and Ki67 was high in 20 cases,while the expression of Ki67 was low in 17 cases.There was a highly positive correlation between the two proteins,which was statistically significant(r=0.879,P < 0.05).Conclusion:1.PHH3 expression was significantly higher in cysteine epithelial carcinoma than in normal urothelial carcinoma;With the increase of clinical stage and pathological grade,the expression level of PHH3 protein increased,suggesting that PHH3 may be involved in the occurrence and development of urinary urothelial carcinoma.2.In non-muscular invasive bladder cancer,patients with positive PHH3 expression have a higher risk of recurrence.3.PHH3 protein level is related to cell proliferation activity,and may be a new prognostic biomarker for urinary urothelial carcinoma.High expression of PHH3 protein is an indicator of poor prognosis in urothelial carcinoma of the bladder.