| Hepatocellular carcinoma(HCC)is one of the malignant tumors with highest fatality rate.With decades of development,a comprehensive treatment system focusing on surgery has gradually formed.However,the 5-year survival rate did not have further improvement in the last 20 years.High rate of recurrence and metastasis after hepatectomy account for the majority death cases,therefore,to explore the molecular mechanism of HCC metastasis and recurrence is significant in the basic research of HCC.RNA binding proteins(RBPs)could bind to target RNA and perform their molecular functions.The disorder of RBPs may participating in the genesis and development of tumors.However,there is no convincible evidence disclosing the functions of RBPs in HCC thoroughly and systematically.In this study,we constructed a RBPs-related prognostic model in HCC based on The Cancer Genome Atlas(TCGA)database.In this model,SNRPA was highly expressed in HCC patients and high expression of SNRPA was negatively associated with prognosis.We constructed knockdown and overexpression of SNRPA HCC cell lines and conducted correlation analysis of biological behavior of tumors.The results showed that SNRPA activated the Wnt / β-catenin signaling pathway of NEAT1 by inhibiting the proteasomal pathway degradation of the YBX3,which is an alternative splicing factor,and ultimately promoted the proliferation,invasion and metastasis of HCC cells.In conclusion,we constructed a RBPs-related prognostic model of HCC,and first disclosing the mechanism SNRPA in the development of HCC.More importantly,this study may provide ideas for developing new strategies in clinical practice in the future. |
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