The Effect Of FASN On The Progression Of Cervical Cancer

Cervical cancer,as the fourth most fatal tumor type among women in the world,is one of the important factors that threaten women’s health.Although the level of prevention and treatment of cervical cancer has been increasing,but the symptoms and signs of cervical cancer patients are often late to appear,and the treatment effect of the advanced patients was significantly lower than that of the early patients,as well as the poor prognosis after treatment makes the low survival rate of patients making the treatment of patients with cervical cancer have considerable difficulty.Therefore,it is of great significance to study the molecular mechanism of the occurrence and development of cervical cancer for the treatment of cervical cancer and to provide new drug targets.In order to understand the genes that have an important influence on the occurrence and development of cervical cancer,this study first searched for the genes that are differentially expressed in cervical cancer tissues through GEPIA,and it was found that the expressions of fatty acid synthase（FASN）in cervical cancer tissues were significantly different from those in normal cervical tissues.FASN,as an important enzyme catalyzing de novo synthesis of fatty acids in cells,is overexpressed in a variety of tumor cells and is closely related to the poor prognosis of patients.This study further obtained various clinical parameters of cervical cancer patients through the TCGA tumor genome database,and used web tools such as THPA and UCSC Xena to analyze the FASN expression of cervical cancer patients and the relationship with the patient’s prognosis,and found that they exist in cervical cancer tissues.The overexpression of FASN is closely related to the poor prognosis of patients with cervical cancer,and the high expression of FASN affects the tumor stage and survival of patients.In order to verify the expression of FASN in cervical cancer tissues,this study carried out immunohistochemistry experiments.Through the analysis of the results of cervical cancer tissue chip immunohistochemistry experiments,it was found that the expression of FASN in cervical cancer tissues was significantly higher than that in adjacent tissues.The high expression of FASN is usually related to the low survival rate of patients,and the up-regulation of FASN expression in cervical cancer cells is closely related to the malignant progression of cancer.In order to further study the expression of FASN in cervical cancer cells and its influence on the biological behavior of cells,the expression level of FASN was detected in Ha Ca T,Siha,Caski,C33 A and He La cells in this study,and the results show that FASN is overexpressed in cervical cancer cells.In cervical cancer cell line He La,the effect of FASN on the proliferation,migration,apoptosis and cycle analyzed after si RNA knockdown of FASN expression,and the results showed that the reduction of FASN expression could significantly inhibit the proliferation and migration of He La cells.Programmed death receptor 1/ programmed death ligand 1（PD-1/PD-L1）is a costimulatory molecule required for T cell activation.A variety of tumor cells evade immunosurveillance by overexpressing or preventing the degradation of PD-L1.FASN expression-related biosynthesis analysis found that FASN expression affects the expression of PD-L1 in cervical cancer tissues.Immunohistochemical experiments also confirmed that FASN expression in cervical cancer tissues can increase the expression of PD-L1.However,the effect of FASN on the expression of PD-L1 was not detected after knocking down the expression of FASN by si RNA in the cervical cancer cell Ca Ski.This study preliminarily found that FASN is overexpressed in cervical cancer and is closely related to the poor prognosis of cervical cancer patients.This effect may be achieved by affecting the level of PD-L1 in cervical cancer tissues.These findings may provide a new idea for further understanding the molecular mechanism of the occurrence and development of cervical cancer and a new target for clinical treatment of cervical cancer.