Expression Level And Clinical Significance Of LncRNA TUG1 In Peripheral Blood Of Patients With Coronary Stent Restenosis

Objective: Percutaneous coronary intervention（PCI）is an effective and commonly used treatment to solve coronary artery stenosis in patients with coronary heart disease（CHD）.In-stent restenosis is a serious complication after stent implantation.ISR seriously affects the daily work and life of CHD patients.Therefore,it is of great significance to explore the pathogenesis and risk factors of ISR and predict the occurrence of ISR in advance.In this study,q RT-PCR was used to detect the relative expression of lnc RNA TUG1 in peripheral blood of patients with in-stent restenosis and non-in-stent restenosis.By comparing the differences in lnc RNA TUG1 expression levels between the two groups of patients,the role of lnc RNA TUG1 in the development of ISR and its reference value as a novel biomarker for predicting ISR were then assessed.To deeply investigate the pathogenesis and clinical significance of TUG1 in ISR.Methods: Patients who were hospitalized in the Department of Cardiology of the Affiliated Hospital of Jining Medical University from July 2019 to June 2021 and underwent coronary angiography review after stent implantation were selected as experimental subjects.According to the interpretation of CAG results by professional interventional physicians,patients with in-stent restenosis were continuously selected as ISR group（n=31）,and patients without in-stent restenosis were randomly selected as N-ISR group（n=31）.Peripheral blood of the enrolled patients was collected,monocytes were isolated,and RNA was extracted from the above cells.Then,q RT-PCR was used to detect the expression level of Lnc RNA TUG1 and evaluate whether the difference was meaningful.Independent risk factors for ISR were determined by logistic analysis.ROC curve was used to evaluate the value of TUG1 for early diagnosis of ISR,and the sensitivity and specificity of TUG1 for diagnosis of ISR were calculated.Results: 1.The expression of TUG1 in the ISR group was significantly greater than that in the N-ISR group（0.1853 ± 0.211 vs 0.0703± 0.0799）.（P<0.05）.2.Smoking,TUG1,and diabetes were found to be independent risk factors for ISR by multiple logistic regression analysis.（P<0.05）.3.The predictive value of TUG1 for ISR was evaluated by calculating the Area Under the ROC Curve（AUC）of TUG1.The AUC of TUG1 was0.707,（95% CI 0.577,0.836）,and the Yuden index was 0.419.The sensitivity and specificity of TUG1 for the diagnosis of ISR were 71.0%and 70.0%,respectively.Conclusion : 1.TUG1 is significantly highly expressed in the peripheral blood of ISR patients,suggesting that TUG1 may play a role in the formation of ISR.TUG1 is highly likely to be involved in the pathogenesis of ISR.2.TUG1 is an independent risk factor for ISR patients and may serve as a novel candidate biomolecular marker for early assessment of ISR after stent implantation.