CSRP2BP/FGFR2/AKT Signaling Pathway Promotes The Proliferation,Invasion And Migration Of Cervical Cancer Cells

Research background: The previous research of the research group found that compared with normal cervical tissue,CSRP2 BP is highly expressed in cervical cancer tissues and cervical cancer Hela,Si Ha,and C33 A cells;overexpression of CSRP2 BP promotes the proliferation,migration and invasion of Hela and C33 A cervical cancer cells,Silence is the opposite;tumor formation experiments and metastasis experiments in nude mice show that CSRP2 BP promotes the proliferation and metastasis ability of cervical cancer Hela cells in vivo.Analyzing the mRNA-Sequencing results of Hela overexpressing CSRP2 BP cells and their control cells,the results of gene differential expression analysis showed that when CSRP2 BP was overexpressed,the expression of EMT-related factor N-cadherin was up-regulated,and the expression of E-cadherin was down-regulated;KEGG signaling pathway showed that,The PI3K/AKT signaling pathway was obviously activated after CSRP2 BP was overexpressed.Objective: To study the molecular mechanism of CSRP2 BP in regulating the proliferation,migration and invasion of cervical cancer cells.Methods: According to the results of the previous mRNA-Sequencing sequencing,the potential target genes of CSRP2 BP in Hela cells were analyzed in Hela cells overexpressing and silenced CSRP2 BP.In Hela cells overexpressing CSRP2 BP,immunofluorescence was used to detect the expression of FGFR2,N-cadherin,and E-cadherin.In Hela cells overexpressing CSRP2 BP,RT-PCR was used to detect the expression of FGFR2,N-cadherin,and E-cadherin.In Hela cells overexpressing CSRP2 BP,Western Blot was used to detect the expression of FGFR2,N-cadherin,E-cadherin,PI3 K,p-PI3 K,AKT,and p-AKT.In Hela cells overexpressing CSRP2 BP,the FGFR2 specific inhibitor RPT835 was used to pretreat the cells.RT-PCR was used to detect the mRNA expression levels of FGFR2,N-cadherin,and E-cadherin in the pretreated cells.Western Blot was used to detect the pretreatment levels.The protein expression levels of FGFR2,N-cadherin,E-cadherin,AKT,and p-AKT in the cells.In the cells pretreated with FGFR2 inhibitors,single-cell plate clone formation experiments,migration experiments,and Transwell experiments were used to evaluate cell proliferation,migration and invasion capabilities.Result:1.Through the analysis of mRNA-Sequencing sequencing results,FGFR2 is a potential target gene of CSRP2 BP in Hela cells.2.In Hela cells overexpressing CSRP2 BP,FGFR2,N-cadherin and E-cadherin are expressed on the cell membrane.3.In Hela cells overexpressing CSRP2 BP,the expression of FGFR2 and N-cadherin was up-regulated,and the expression of E-cadherin was down-regulated.4.In Hela cells overexpressing CSRP2 BP,p-AKT expression is up-regulated.5.In Hela cells overexpressing CSRP2 BP,after inhibiting FGFR2,the expression of N-cadherin was down-regulated,the expression of E-cadherin was up-regulated,and the expression of p-AKT was down-regulated.6.In Hela cells overexpressing CSRP2 BP,after inhibiting FGFR2,the proliferation,migration and invasion ability of Hela cells is weakened.Conclusion:1.CSRP2 BP up-regulates the expression of FGFR2 and N-cadherin,activates the phosphorylation level of AKT,and promotes the proliferation,migration and invasion of cervical cancer Hela cells.2.After inhibiting the expression of FGFR2,the expression of N-cadherin is down-regulated,and the expression of E-cadherin is up-regulated,inhibiting the phosphorylation level of AKT,and inhibiting the proliferation,migration and invasion of cervical cancer Hela cells.