Preparation And In Vitro Study Of TCP/PCL/PTMC Scaffolds Based On Electrospinning

Purposes1.Composite fiber scaffolds containing Polycaprolactone(PCL),Poly(trimethylene carbonate)(PTMC)and different mass percentages of Calcium phosphate tribasic(TCP)were prepared by electrospinning.The modification of pure PCL scaffolds by adding PTMC and TCP was evaluated.2.Study was conduct by co-culture Osteoblasts and TCP/PCL/PTMC composite fiber scaffolds in vitro,to evaluate the biocompatibility of the composite fiber scaffolds and to evaluate the effects of the scaffolds with different calcium phosphate contents on osteoblast osteogenic activity and proliferation.Methods1.PCL/PTMC scaffolds were prepared by electrospinning after blending with polycaprolactone : polytrimethylene carbonate at a mass ratio of 1 : 3 and dissolving into electrospinning solution.In addition,TCP/PCL/PTMC composite scaffolds were prepared by adding 10 wt.%,20 wt.% and 30 wt.% calcium phosphate into the above electrospinning solution.The swelling rate,porosity,degradation rate,surface morphology,hydrophilic angle and mechanical properties of pure PCL scaffolds,PCL/PTMC scaffolds and TCP/PCL/PTMC scaffolds were detected by liquid replacement method,PBS immersion method,scanning electron microscope,hydrophilic angle tester and universal mechanical machine,respectively.2.Osteoblasts were seeded on PCL/PTMC scaffolds and TCP/PCL/PTMC composite scaffolds for co-culture.Then,the proliferation of osteoblasts was detected on the 1st,3rd and 7th day of co-culture,respectively.3.Osteoblasts were co-cultured with PCL/PTMC scaffolds and TCP/PCL/PTMC composite scaffolds,and the activity of alkaline phosphatase(ALP)in osteoblasts was detected by ALP kit on the 3rd and 7th day of co-culture,respectively.Results1.The results of electron microscopy showed that the fiber distribution on the surface of PCL/PTMC scaffolds was uneven and staggered,and the fiber bundles were bonded and fused.And,the fibers on the surface of TCP/PCL/PTMC scaffolds were similar to PCL/PTMC scaffolds,but there were many fiber bundle fractures.TCP/PCL/PTMC scaffolds had better swelling rate,degradation rate,porosity and hydrophilicity than PCL scaffolds and PCL/PTMC scaffolds.PCL/PTMC scaffolds had higher elastic modulus than pure PCL scaffolds(P < 0.05).Although the elastic modulus of TCP/PCL/PTMC scaffolds were lower than PCL/PTMC scaffolds,the max stress at break was significantly increased(P < 0.05).2.In vitro,the proliferation experiments of cell showed that TCP/PCL/PTMC composite scaffolds had stronger effect on promoting osteoblasts proliferation than PCL/PTMC scaffolds(P < 0.05).Among them,the TCP/PCL/PTMC scaffolds with20% calcium phosphate tribasic had strongest promotion effect(P < 0.05).3.The results of alkaline phosphatase activity experiments in vitro showed that the osteogenic activity of osteoblasts co-cultured with TCP/PCL/PTMC composite scaffolds was higher than PCL/PTMC scaffolds(P < 0.05).And,the TCP/PCL/PTMC scaffolds containing 20% calcium phosphate tribasic had the highest osteogenic activity of osteoblasts(P < 0.05).Conclusions1.The TCP/PCL/PTMC composite scaffolds prepared by electrospinning have better degradation rate,porosity,hydrophilicity and mechanical properties.2.The TCP/PCL/PTMC composite scaffolds can enhance the osteogenic activity of osteoblasts and promote the proliferation of osteoblasts.3.The TCP/PCL/PTMC composite scaffolds with 20% calcium phosphate tribasic have excellent biocompatibility,bioactivity and mechanical properties,which is an ideal scaffold to promote tendon-to-bone healing.