Therapeutic Effect And Mechanism Of Shu Xin Sheng Mai Dan On Rat Model Of Myocardial Ischemia With Coronary Ligation

Objective: To investigate the therapeutic effect of Shuxinshengmai Dan on myocardial ischemia rat model by coronary artery ligation and to clarify its potential mechanism through PI3 K / AKT / FOXO1 signal pathway.Method: 1.70 adult SPF SD rats were randomly divided into control group,sham group,model group,positive control group,Shuxinshengmai Dan low-dose group,middle-dose group and high-dose group,continuous intragastric administration for 5 days.A rat model of myocardial ischemia was established by ligating the left anterior descending branch of the coronary artery.2.After 5 days,the right carotid artery was intubated to detect the changes of Hemodynamics and the serum creatine Isoenzyme(CK-MB)was detected by enzyme-linked immunoassay(ELISA).3.HE staining and transmission electron microscopy were used to observe the structural changes of cardiomyocytes,muscle fibers,mitochondria,and myofilaments in rat myocardium.4.The expression of PI3 K,Akt,FOXO1 and Bim m RNA in myocardium was detected by quantitative real-time PCR.5.Western blot was used to detect the expression of PI3 K,Akt,FOXO1,Caspase-3protein in rat heart tissue.Result: 1.Compared with the model group,both the positive control group and the Shuxinshengmai Dan groups had protective effects on the damaged myocardium of rats,and improved the left ventricular function of rats effectively(p < 0.05).2.The serum CK-MB of the rats in the treatment groups was significantly decreased after Shuxinshengmai Dan and compound Danshen dropping pills were administered orally(p < 0.05).3.The results of RT-PCR showed that PI3 K and Akt m RNA in model group were decreased,while FOXO1 and Bim m RNA were up-regulated(p < 0.05).Compared with model group,PI3 K and Akt m RNA in myocardial tissue of treatment groups were increased obviously,while the expression level of FOXO1 and Bim m RNA were down-regulated(p <0.05).4.Western blot results showed that PI3 K and Akt protein were significantly decreased,FOXO1 and Caspase-3 protein were increased in model group compared with control group(p < 0.05).PI3 K and Akt protein were increased in each group after drug pretreatment,FOXO1 and Caspase-3 protein were down-regulated(p < 0.05).Conclusion: Shuxinshengmai Dan can significantly improve cardiac function and inhibit cell apoptosis in myocardial ischemia model rats,which may be mediated by PI3 K /AKT / FOXO1 signaling pathway,which has therapeutic effects on the establishment of MI rat models by coronary artery ligation.