Serum Metabonomics Research Of Children With Rotavirus Infection

Objective: By testing the serum metabolites of rotavirus-infected patients and healthy people based on ultra-performance liquid chromatographyhigh resolution time-of-flight mass spectrometer(UPLC-HRTOFMS),and screening of differential metabolites and abnormal metabolic pathways,in order to look for potential biomarkers of rotavirus infection,and provide new ideas for the diagnosis of rotavirus infection.Methods: 41 patients with rotavirus infection diagnosed by Hunan Provincial People’s Hospital were selected as the experimental group,and37 healthy people as the control group from November 2020 to February2021,and collect fasting serum samples from the two groups.UPLC-HRTOF-MS combined technology was used to detect the serum metabolites of the two groups to obtain metabolic maps and raw data.Use Metabo Analyst 5.0 website for univariate analysis and multivariate statistical analysis to identify changes in each metabolite,and screen differential metabolites [variable important in projection(VIP)>1,fold change(FC)>2 and P<0.05],and further conduct metabolic pathway analysis.Finally,the receiver operating characteristic curve(ROC)screens potential biomarkers.Results:1.A total of 120 differential metabolites were screened through univariate analysis(FC>2,P<0.05),and 33 differential metabolites were screened out through multivariate statistical analysis combined with VIP>1,FC>2 and P<0.05,including 7 metabolites such as pentosidin,glycerophosphocholine were down-regulated,and 26 metabolites such as pyruvate and glucose 6-phosphate were up-regulated.2.Five metabolic pathways include cysteine and methionine metabolism,starch and sucrose metabolism,nicotinate and nicotinamide metabolism,pyruvate metabolism and glycolysis / gluconeogenesismay be involved in the metabolic network of rotavirus infection.3.The ROC curve analysis of the differential metabolites showed that the AUC>0.9 of 9 metabolites.Among them,the metabolite corresponding to the highest AUC value was adrenaline ethanolamide.Conclusion: 1.There are significant differences in the metabolites of rotavirus-infected patients and healthy people.2.33 metabolites such as pentosidin,pyruvate,and glucose 6-phosphate are abnormally expressed during rotavirus infection.3.Rotavirus infection may lead to abnormal pathways in cysteine and methionine metabolism,starch and sucrose metabolism,nicotinate and nicotinamide metabolism,pyruvate metabolism,and glycolysis /gluconeogenesis pathways.4.Adrenoyl ethanolamide may be potential biomarkers of rotavirus infection.